Department of Biology, Allegheny College, Meadville, Pennsylvania 16335.
Plant Physiol. 1985 Dec;79(4):1129-32. doi: 10.1104/pp.79.4.1129.
Corn (Zea mays L., W23(N), OH43(N), and reciprocal single cross hybrid) seedling mitochondria respond to a 10 degrees C temperature shift (27-37 degrees C) by incorporating a greater amount of [(35)S]methionine into acid-insoluble material than mitochondria incubated at the original growing temperature (27 degrees C). This increase is in part manifested in the enhanced synthesis of a 52 kilodaltons protein. At both temperatures mitochondria of two inbreds and their reciprocal hybrids synthesize normal (N) cytoplasm proteins sensitive to chloramphenicol and insensitive to cyclohexamide treatment. The 52 kilodaltons protein is found in the supernatants of pelleted (15,000g, 5 min) mitochondria after heat shock. The role of this protein in the heat shock response is discussed in light of the implication of mitochondria as the primary cellular target to temperature stress.
玉米（Zea mays L.，W23（N），OH43（N）和相互单交杂种）幼苗线粒体对 10°C 的温度变化（27-37°C）的反应是比在原始生长温度（27°C）下孵育的线粒体掺入更多的[(35)S]蛋氨酸到酸不溶性物质中。这种增加部分表现在增强合成 52 千道尔顿的蛋白质。在两种自交系及其相互杂种的线粒体中，两种温度下均合成对氯霉素敏感而对环己酰胺处理不敏感的正常（N）细胞质蛋白。52 千道尔顿的蛋白质在热休克后沉淀（15,000g，5min）的线粒体上清液中被发现。考虑到线粒体作为细胞对温度应激的主要靶标，讨论了该蛋白在热休克反应中的作用。
