Centro de Investigación sobre Fijación de Nitrógeno, U.N.A.M., Cuernavaca, Mor. Apartado Postal 565-A, México.
Plant Physiol. 1986 Apr;80(4):1051-4. doi: 10.1104/pp.80.4.1051.
Immunoprecipitation and two-dimensional gel electrophoresis analysis of the glutamine synthetase (GS) polypeptides (alpha and beta) during Phaseolus vulgaris root development shows that the alpha polypeptide is the main component of the enzyme in the embryo and in up to 5 day old roots. From 5 days on, the beta polypeptide becomes the root predominant GS monomer. The alpha/beta ratio of the in vitro translated GS polypeptides from the total polysomal RNA isolated at different root ages correlates with the alpha/beta ratio observed in the root extracts. These results suggest that the two root GS polypeptides are encoded by different mRNA species in Phaseolus vulgaris.
免疫沉淀和二维凝胶电泳分析菜豆根发育过程中的谷氨酰胺合成酶（GS）多肽（α和β）表明，α多肽是胚胎和 5 天内根中酶的主要成分。从第 5 天开始，β多肽成为根中主要的 GS 单体。从不同根龄分离的总多核糖体 RNA 体外翻译的 GS 多肽的α/β比值与根提取物中观察到的α/β比值相关。这些结果表明，菜豆的两种根 GS 多肽是由不同的 mRNA 种类编码的。
