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菜豆和利马豆根瘤中谷氨酰胺合成酶的同工酶

Isozymes of Glutamine Synthetase in Phaseolus vulgaris L. and Phaseolus lunatus L. Root Nodules.

作者信息

Robert F M, Wong P P

机构信息

Division of Biology, Kansas State University, Manhattan, Kansas 66506.

出版信息

Plant Physiol. 1986 May;81(1):142-8. doi: 10.1104/pp.81.1.142.

DOI:10.1104/pp.81.1.142
PMID:16664764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1075297/
Abstract

The glutamine synthetase (GS) isozymes in the plant fraction of nodule extracts from 62 cultivars of Phaseolus vulgaris L. and one cultivar of Phaseolus lunatus L. were analyzed by polyacrylamide gel electrophoresis. All P. vulgaris nodule extracts displayed two GS activity bands: a nodule-specific band (GS(n1)) and a band (GS(n2)) similar to the single band (GS(r)) present in root extracts. In nodule extracts of P. lunatus, the GS(n1) band was detected, but the GS(n2) band was barely detectable. In contrast to P. vulgaris, the GS(n2) band and the GS(r) band of P. lunatus appeared to be different. The electrophoretic mobility of the GS(n1) band in P. vulgaris was governed by both the plant cultivar and the development stage of the nodule. In nodule extracts of P. vulgaris and P. lunatus, the zone of GS(n1) activity coincided with six to nine distinct protein bands as revealed after treatment of gels, which had previously been stained for GS activity, with Coomassie blue. All these protein bands were shown to consist of polypeptides of identical molecular weight (approximately 47,000 daltons) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our results indicate that P. vulgaris continuously generates isozymes of GS(n1) of increasing electrophoretic mobility during the course of nodule development.

摘要

通过聚丙烯酰胺凝胶电泳分析了62个菜豆品种和1个利马豆品种根瘤提取物植物部分中的谷氨酰胺合成酶（GS）同工酶。所有菜豆根瘤提取物均显示出两条GS活性带：一条根瘤特异性带（GS(n1)）和一条与根提取物中存在的单一条带（GS(r)）相似的带（GS(n2)）。在利马豆的根瘤提取物中，检测到了GS(n1)带，但GS(n2)带几乎检测不到。与菜豆不同，利马豆的GS(n2)带和GS(r)带似乎不同。菜豆中GS(n1)带的电泳迁移率受植物品种和根瘤发育阶段的影响。在用考马斯亮蓝处理先前已进行GS活性染色的凝胶后发现，菜豆和利马豆根瘤提取物中GS(n1)活性区域与6至9条不同的蛋白带重合。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳表明，所有这些蛋白带均由分子量相同（约47,000道尔顿）的多肽组成。我们的结果表明，菜豆在根瘤发育过程中持续产生电泳迁移率不断增加的GS(n1)同工酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/ad6524f3148f/plntphys00601-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/061c503038da/plntphys00601-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/164a9aad865e/plntphys00601-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/560362c53a28/plntphys00601-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/6236478ce228/plntphys00601-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/fc927348093f/plntphys00601-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/ad6524f3148f/plntphys00601-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/061c503038da/plntphys00601-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/164a9aad865e/plntphys00601-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/560362c53a28/plntphys00601-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/6236478ce228/plntphys00601-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/fc927348093f/plntphys00601-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c834/1075297/ad6524f3148f/plntphys00601-0154-a.jpg

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本文引用的文献

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