焦磷酸果糖 6-磷酸磷酸转移酶在玉米叶片中的分布。
Distribution of pyrophosphate:fructose 6-phosphate phosphotransferase in maize leaves.
机构信息
Biology Department, Queen's University, Kingston, Ontario, Canada K7L 3N6.
出版信息
Plant Physiol. 1986 May;81(1):12-6. doi: 10.1104/pp.81.1.12.
Abstract
In leaves of maize (Zea mays) the activity of pyrophosphate:fructose 6-phosphate 1-phosphotransferase (PFP) is much less than that of ATP:fructose 6-phosphate 1-phosphotransferase. A sequential extraction technique was used to study the location of PFP in this tissue. When compared with enzymes known to be restricted to specific locations in maize, the distribution of PFP activity in the sequential extracts indicated that PFP is located predominantly, if not exclusively, in the mesophyll cytoplasm. Although confined to the same site as sucrose synthesis, the level of PFP activity is inadequate to contribute significantly to the gluconeogenic flux from fructose 1,6-bisphosphate to fructose 6-phosphate. The absence of PFP activity from the bundle-sheath demonstrates that this activity is not essential for glycolysis in higher plants.
摘要
在玉米（Zea mays）的叶片中，焦磷酸：果糖 6-磷酸 1-磷酸转移酶（PFP）的活性远低于 ATP：果糖 6-磷酸 1-磷酸转移酶。本文采用顺序提取技术研究了该组织中 PFP 的位置。与已知局限于玉米特定位置的酶相比，PFP 活性在顺序提取物中的分布表明，PFP 主要（如果不是唯一）位于叶肉细胞质中。尽管 PFP 与蔗糖合成位于同一部位，但它的活性不足以对果糖 1,6-二磷酸到果糖 6-磷酸的糖异生通量产生显著贡献。鞘细胞中不存在 PFP 活性表明，该活性对于高等植物的糖酵解并非必需。
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本文引用的文献
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