1-氨基环丙烷-1-羧酸与次氯酸盐试剂检测法的干扰因素及特异性
Interferences and specificity of the 1-aminocyclopropane-1-carboxylic Acid assay with the hypochlorite reagent.
作者信息
Nieder M, Yip W K, Yang S F
机构信息
Department of Vegetable Crops, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1986 May;81(1):156-60. doi: 10.1104/pp.81.1.156.
1-Aminocyclopropane-1-carboxylic Acid (ACC), the immediate precursor of ethylene is routinely assayed by converting it into ethylene with NaOCl, and the ethylene liberated is then determined by gas chromatography (MCC Lizada, SF Yang 1979 Anal Biochem 100: 140-145). However, certain materials which may be present in crude plant extracts or in enzyme reaction mixtures interfere with this assay procedure. Mono, and di-alkyl amines cause poor yields of ethylene from ACC. Ethanol in the presence of NH(3) or amines but in the absence of ACC can produce ethylene under the assay procedure. The characteristics of these interfering reactions were studied and precautions to avoid these problems are suggested. Recovery of ACC during its extraction and purification from plant extracts were tested and are discussed.
1-氨基环丙烷-1-羧酸（ACC）是乙烯的直接前体，通常通过用次氯酸钠将其转化为乙烯来进行测定，然后通过气相色谱法测定释放出的乙烯（MCC·利扎达、SF·杨，1979年，《分析生物化学》100：140 - 145）。然而，粗植物提取物或酶反应混合物中可能存在的某些物质会干扰该测定程序。单烷基胺和二烷基胺会导致ACC生成乙烯的产率较低。在有氨或胺存在但没有ACC的情况下，乙醇在测定程序中会产生乙烯。研究了这些干扰反应的特性，并提出了避免这些问题的预防措施。测试并讨论了从植物提取物中提取和纯化ACC过程中的回收率。
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