Hosler J P, Yocum C F
Department of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048.
Plant Physiol. 1987 Apr;83(4):965-9. doi: 10.1104/pp.83.4.965.
Addition of ferredoxin to isolated thylakoid membranes reconstitutes electron transport from water to NADP and to O(2) (the Mehler reaction). This electron flow is coupled to ATP synthesis, and both cyclic and noncyclic electron transport drive photophosphorylation. Under conditions where the NADPH/NADP(+) ratio is varied, the amount of ATP synthesis due to cyclic activity is also varied, as is the amount of cyclic activity which is sensitive to antimycin A. Partial inhibition of photosystem II activity with DCMU (which affects reduction of electron carriers of the interphotosystem chain) also affects the level of cyclic activity. The results of these experiments indicate that two modes of cyclic electron transfer activity, which differ in their antimycin A sensitivity, can operate in the thylakoid membrane. Regulation of these activities can occur at the level of ferredoxin and is governed by the NADPH/NADP ratio.
向分离的类囊体膜中添加铁氧化还原蛋白可重建从水到NADP以及到O₂（梅勒反应）的电子传递。这种电子流与ATP合成相偶联，并且循环和非循环电子传递都驱动光合磷酸化。在NADPH/NADP⁺比值变化的条件下，由于循环活性导致的ATP合成量也会变化，对抗霉素A敏感的循环活性量也是如此。用二氯苯基二甲基脲（DCMU）部分抑制光系统II活性（这会影响光系统间链中电子载体的还原）也会影响循环活性水平。这些实验结果表明，两种对抗霉素A敏感性不同的循环电子传递活性模式可在类囊体膜中运行。这些活性的调节可发生在铁氧化还原蛋白水平，并受NADPH/NADP比值控制。
