通过酶免疫测定法使用VP7特异性单克隆抗体对猪轮状病毒进行直接血清分型。

Direct serotyping of porcine rotaviruses using VP7-specific monoclonal antibodies by an enzyme immunoassay.

作者信息

Nagesha H S, Holmes I H

机构信息

School of Microbiology, University of Melbourne, Parkville, Victoria, Australia.

出版信息

J Med Virol. 1991 Nov;35(3):206-11. doi: 10.1002/jmv.1890350311.

DOI:10.1002/jmv.1890350311

PMID:1666648

Abstract

Employing a serotyping EIA test using MAbs both cell culture adapted and faecal porcine rotaviruses were classified into serotypes G3, G3/5, G4, and G5. The MAbs have confirmed and extended the serotyping results obtained using polyclonal antisera. These MAbs are therefore potential reagents for serotyping of porcine rotaviruses. Using subgroup specific MAbs serotypes G3, G3/5, and G5 were found to contain subgroup I antigens while G4 rotaviruses contained either subgroup II or subgroup I antigens.

摘要

利用一种使用单克隆抗体的血清分型酶免疫分析试验，细胞培养适应型和粪便猪轮状病毒均被分为G3、G3/5、G4和G5血清型。这些单克隆抗体证实并扩展了使用多克隆抗血清获得的血清分型结果。因此，这些单克隆抗体是猪轮状病毒血清分型的潜在试剂。使用亚组特异性单克隆抗体发现，G3、G3/5和G5血清型含有I亚组抗原，而G4轮状病毒含有II亚组或I亚组抗原。