Varner J E, Taylor R
Institute of Molecular Biology, Academia Sinica, Nankang Taipei, 11529 Republic of China.
Plant Physiol. 1989 Sep;91(1):31-3. doi: 10.1104/pp.91.1.31.
We report the use of Ni(2+) and Co(2+) on free-hand sections of soybean (Glycine max L.) and Bidens sp to localize polygalacturonates. In soybean only the hourglass cells of the seedcoat stain intensely. In the pod the epidermis of the outer pod wall and a few layers of subepidermal cells stain lightly, while that part of the funiculus adjacent to the seedcoat palisade epidermal cells stains heavily and the neck of the funiculus close to the pod also stains. In Bidens stem sections, the walls of the collenchyma stain most intensely.
我们报道了使用镍离子（Ni(2+)）和钴离子（Co(2+)）对大豆（Glycine max L.）和鬼针草属植物的徒手切片进行处理，以定位多聚半乳糖醛酸酯。在大豆中，只有种皮的沙漏状细胞染色强烈。在豆荚中，外豆荚壁的表皮和几层皮下细胞染色较浅，而与种皮栅栏表皮细胞相邻的珠柄部分染色很深，靠近豆荚的珠柄颈部也染色。在鬼针草茎切片中，厚角组织的细胞壁染色最强烈。
