Moore P J, Darvill A G, Albersheim P, Staehelin L A
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347.
Plant Physiol. 1986 Nov;82(3):787-94. doi: 10.1104/pp.82.3.787.
they impart rigidity to the plant, provide a physical and chemical barrier between the cell and its environment, and regulate the size and shape of each cell. Chemical studies have provided information on the biochemical composition of the plant cell walls as well as detailed knowledge of individual cell wall molecules. In contrast, very little is known about the distribution of specific cell wall components around individual cells and throughout tissues. To address this problem, we have produced polyclonal antibodies against two cell wall matrix components; rhamnogalacturonan I (RG-I), a pectic polysaccharide, and xyloglucan (XG), a hemicellulose. By using the antibiodies as specific markers we have been able to localize these polymers on thin sections of suspension-cultured sycamore cells (Acer pseudoplatanus). Our results reveal that each molecule has a unique distribution. XG is localized throughout the entire wall and middle lamella. RG-I is restricted to the middle lamella and is especially evident in the junctions between cells. These observations indicate that plant cell walls may have more distinct chemical (and functional?) domains than previously envisaged.
它们赋予植物刚性，在细胞与其环境之间提供物理和化学屏障，并调节每个细胞的大小和形状。化学研究提供了有关植物细胞壁生化组成的信息以及对单个细胞壁分子的详细了解。相比之下，关于特定细胞壁成分在单个细胞周围和整个组织中的分布却知之甚少。为了解决这个问题，我们制备了针对两种细胞壁基质成分的多克隆抗体；鼠李半乳糖醛酸聚糖I（RG-I），一种果胶多糖，以及木葡聚糖（XG），一种半纤维素。通过使用这些抗体作为特异性标记，我们能够将这些聚合物定位在悬浮培养的悬铃木细胞（槭树）的薄片上。我们的结果表明，每个分子都有独特的分布。XG分布在整个细胞壁和胞间层。RG-I局限于胞间层，在细胞间连接处尤为明显。这些观察结果表明，植物细胞壁可能具有比以前设想的更明显的化学（和功能？）区域。
