Unidad de Biotecnología, Instituto Internacional de Estudios Avanzados, Apartado 17606, Caracas 1015A, Venezuela.
Plant Physiol. 1991 Jan;95(1):335-6. doi: 10.1104/pp.95.1.335.
Cassava (Manihot esculenta Crantz) protoplast was analyzed by using isoelectric focusing techniques. Two populations, representing 68 and 32% of the total sample, with mean isoelectric points of 4.48 and 4.60, were obtained using mesophyll protoplasts. The use of this technique allows demonstration of a discontinuous distribution of protoplast isoelectric point from one species according to their surface potential.
利用等电聚焦技术分析木薯(Manihot esculenta Crantz)原生质体。使用叶肉原生质体获得了两个群体,分别代表总样本的 68%和 32%,平均等电点为 4.48 和 4.60。该技术的使用证明了根据原生质体表面电势,其等电点呈不连续分布。