Ait Barka Essaid, Nowak Jerzy, Clément Christophe
Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UPRES EA 2069, UFR Sciences, Université de Reims Champagne-Ardenne, 51687 Reims Cédex 2, France.
Appl Environ Microbiol. 2006 Nov;72(11):7246-52. doi: 10.1128/AEM.01047-06. Epub 2006 Sep 15.
In vitro inoculation of Vitis vinifera L. cv. Chardonnay explants with a plant growth-promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, increased grapevine growth and physiological activity at a low temperature. There was a relationship between endophytic bacterial colonization of the grapevine plantlets and their growth at both ambient (26 degrees C) and low (4 degrees C) temperatures and their sensitivities to chilling. The major benefits of bacterization were observed on root growth (11.8- and 10.7-fold increases at 26 degrees C and 4 degrees C, respectively) and plantlet biomass (6- and 2.2-fold increases at 26 degrees C and 4 degrees C, respectively). The inoculation with PsJN also significantly improved plantlet cold tolerance compared to that of the nonbacterized control. In nonchilled plantlets, bacterization enhanced CO(2) fixation and O(2) evolution 1.3 and 2.2 times, respectively. The nonbacterized controls were more sensitive to exposure to low temperatures than were the bacterized plantlets, as indicated by several measured parameters. Moreover, relative to the noninoculated controls, bacterized plantlets had significantly increased levels of starch, proline, and phenolics. These increases correlated with the enhancement of cold tolerance of the grapevine plantlets. In summary, B. phytofirmans strain PsJN inoculation stimulates grapevine growth and improves its ability to withstand cold stress.
用促生根际细菌伯克霍尔德氏菌PsJN菌株对酿酒葡萄品种霞多丽外植体进行体外接种，可在低温下促进葡萄生长并提高其生理活性。葡萄幼苗的内生细菌定殖与其在环境温度（26℃）和低温（4℃）下的生长以及对低温的敏感性之间存在关联。细菌接种的主要益处体现在根系生长（在26℃和4℃时分别增加11.8倍和10.7倍）和幼苗生物量（在26℃和4℃时分别增加6倍和2.2倍）方面。与未接种细菌的对照相比，接种PsJN还显著提高了幼苗的耐寒性。在未受低温胁迫的幼苗中，细菌接种分别使二氧化碳固定和氧气释放增强了1.3倍和2.2倍。如多个测量参数所示，未接种细菌的对照比接种细菌的幼苗对低温暴露更敏感。此外，相对于未接种的对照，接种细菌的幼苗中淀粉、脯氨酸和酚类物质的含量显著增加。这些增加与葡萄幼苗耐寒性的增强相关。总之，接种伯克霍尔德氏菌PsJN菌株可刺激葡萄生长并提高其抵御冷胁迫的能力。
