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花生中两个II类几丁质酶基因的特性分析及在转基因烟草植株中的表达研究

Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants.

作者信息

Kellmann J W, Kleinow T, Engelhardt K, Philipp C, Wegener D, Schell J, Schreier P H

机构信息

Max-Planck-Institut für Züchtungsforschung, Köln, FRG.

出版信息

Plant Mol Biol. 1996 Jan;30(2):351-8. doi: 10.1007/BF00020121.

DOI:10.1007/BF00020121
PMID:8616259
Abstract

Two different genes encoding class II chitinases from peanut (Arachis hypogaea L. cv. NC4), A.h.Chi2;1 and A.h.Chi2;2, have been cloned. In peanut cell suspension cultures, mRNA levels of A.h.Chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from Botrytis cinerea. The second gene, A.h.Chi2;1, was only expressed after treatment with the fungal spores. Transgenic tobacco plants containing the complete peanut A.h.Chi2;1 gene exhibited essentially the same expression pattern in leaves as observed in peanut cell cultures. Expression characteristics of transgenic tobacco carrying a promoter-GUS fusion of A.h.Chi2;1 are described.

摘要

已克隆出花生（Arachis hypogaea L. cv. NC4）中编码II类几丁质酶的两个不同基因，即A.h.Chi2;1和A.h.Chi2;2。在花生细胞悬浮培养物中，乙烯或水杨酸处理后以及在灰葡萄孢分生孢子存在的情况下，A.h.Chi2;2的mRNA水平会升高。第二个基因A.h.Chi2;1仅在真菌孢子处理后表达。含有完整花生A.h.Chi2;1基因的转基因烟草植株在叶片中的表达模式与在花生细胞培养物中观察到的基本相同。本文描述了携带A.h.Chi2;1启动子-GUS融合基因的转基因烟草的表达特性。

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本文引用的文献

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Release of highly elicitor-active glucans by germinating zoospores of Phytophthora megasperma f. sp. glycinea.由大豆疫霉游动孢子萌发释放高激发活性的葡聚糖。
Planta. 1992 Nov;188(4):498-505. doi: 10.1007/BF00197041.
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A simple and general method for transferring genes into plants.一种将基因转入植物的简单而通用的方法。
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Regulation of a chitinase gene promoter by ethylene and elicitors in bean protoplasts.豆原细胞中乙烯和诱导子对几丁质酶基因启动子的调控。
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Over-expression of a cacao class I chitinase gene in Theobroma cacao L. enhances resistance against the pathogen, Colletotrichum gloeosporioides.可可树I类几丁质酶基因在可可中的过表达增强了对病原菌胶孢炭疽菌的抗性。
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Planta. 2006 Feb;223(3):433-48. doi: 10.1007/s00425-005-0099-6. Epub 2005 Sep 6.
6
Members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls.一组新的几丁质酶样基因成员在具有次生壁的棉花细胞中优先表达。
Plant Mol Biol. 2004 Feb;54(3):353-72. doi: 10.1023/B:PLAN.0000036369.55253.dd.
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Xylanase, a novel elicitor of pathogenesis-related proteins in tobacco, uses a non-ethylene pathway for induction.木聚糖酶是一种新型的烟草病程相关蛋白诱导剂，它使用非乙烯途径进行诱导。
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