Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1985 Sep;79(1):118-25. doi: 10.1104/pp.79.1.118.
Cell lines of tobacco (Nicotiana tabacum L. var Wisconsin 38) were obtained which are adapted to grow in media with varying concentrations of NaCl, up to 35 grams per liter (599 millimolar). Salt-adapted cells exhibited enhanced abilities to gain both fresh and dry weight in the presence of NaCl compared to cells which were growing in medium without NaCl (unadapted cells). Tolerance of unadapted cells and cells adapted to 10 grams per liter NaCl was influenced by the stage of growth, with the highest degree of tolerance exhibited by cells in the exponential phase. Cell osmotic potential and turgor varied through the growth cycle of unadapted cells and cells at all levels of adaptation, with maximum turgor occurring at approximately the onset of exponential fresh weight accumulation.Adaptation to NaCl led to reduced cell expansion and fresh weight gain, while dry weight gain remained unaffected. This reduction in cell expansion was not due to failure of the cells to maintain turgor since cells adapted to NaCl underwent osmotic adjustment in excess of the change in water potential caused by the addition of NaCl to the medium. Tolerance of the adapted cells, as indicated by fresh or dry weight gain, did not increase proportionately with the increase in turgor. Adaptation of these glycophytic cells to NaCl appears to involve mechanisms which result in an altered relationship between turgor and cell expansion.
烟草（Nicotiana tabacum L. var Wisconsin 38）细胞系适应了在含有不同浓度 NaCl 的培养基中生长，最高可达 35 克/升（599 毫摩尔）。与在不含 NaCl 的培养基中生长的细胞（未适应细胞）相比，适应盐的细胞在有 NaCl 的情况下具有更强的增加鲜重和干重的能力。未适应细胞和适应 10 克/升 NaCl 的细胞的耐盐性受生长阶段的影响，指数生长期细胞的耐盐性最高。未适应细胞和适应不同 NaCl 浓度的细胞的细胞渗透压势和膨压在整个生长周期中发生变化，最大膨压出现在指数期鲜重积累的开始时。适应 NaCl 导致细胞扩张和鲜重增加减少，而干重增加不受影响。这种细胞扩张减少不是由于细胞不能维持膨压所致，因为适应 NaCl 的细胞在培养基中添加 NaCl 引起的水势变化之外进行了渗透调节。适应细胞的耐盐性（如鲜重或干重增加所示）并没有随膨压的增加成比例增加。这些糖质细胞对 NaCl 的适应似乎涉及导致膨压和细胞扩张之间关系改变的机制。
