Larosa P C, Handa A K, Hasegawa P M, Bressan R A
Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1985 Sep;79(1):138-42. doi: 10.1104/pp.79.1.138.
Adaptation of tobacco (Nicotiana tabacum L. var Wisconsin 38) cells to NaCl was accelerated by (+/-) abscisic acid (ABA). In medium with 10 grams per liter NaCl, ABA stimulated the growth of cells not grown in medium with NaCl (unadapted, S-0) with an increasing response from 10(-8) to 10(-4) molar. ABA (10(-5) molar) enhanced the growth of unadapted cells in medium with 6 to 22 grams per liter NaCl but did not increase the growth of cells previously adapted to either 10 (S-10) or 25 (S-25) grams per liter NaCl unless the cells were inoculated into medium with a level of NaCl higher than the level to which the cells were adapted. The growth of unadapted cells in medium with Na(2)SO(4) (85.5 millimolar), KCl (85.5 or 171 millimolar), K(2)SO(4) (85.5 millimolar) was also stimulated by ABA. ABA (10(-8)-10(-4) molar) did not accelerate the growth of unadapted cells exposed to water deficits induced by polyethylene glycol (molecular weight 8000) (5-20 grams per 100 milliliters), sorbitol (342 millimolar), mannitol (342 millimolar) or sucrose (342 millimolar). These results suggest that ABA is involved in adaptation of cells to salts, and is not effective in promoting adaptation to water deficits elicited by nonionic osmotic solutes.
（±）脱落酸（ABA）可加速烟草（Nicotiana tabacum L. var Wisconsin 38）细胞对氯化钠的适应。在含有每升10克氯化钠的培养基中，ABA刺激了未在含氯化钠培养基中生长的细胞（未适应，S-0）的生长，其响应随着浓度从10^(-8) 摩尔增加到10^(-4) 摩尔而增强。ABA（10^(-5) 摩尔）增强了未适应细胞在含有每升6至22克氯化钠的培养基中的生长，但除非将细胞接种到氯化钠水平高于其适应水平的培养基中，否则不会增加先前适应每升10克（S-10）或25克（S-25）氯化钠的细胞的生长。ABA也刺激了未适应细胞在含有硫酸钠（85.5毫摩尔）、氯化钾（85.5或171毫摩尔）、硫酸钾（85.5毫摩尔）的培养基中的生长。ABA（10^(-8)-10^(-4) 摩尔）不会加速暴露于由聚乙二醇（分子量8000）（每100毫升5至20克）、山梨醇（342毫摩尔）、甘露醇（342毫摩尔）或蔗糖（342毫摩尔）引起的水分亏缺下的未适应细胞的生长。这些结果表明，ABA参与细胞对盐的适应，而在促进对非离子渗透溶质引起的水分亏缺的适应方面无效。
