利用同位素稀释气相色谱-质谱分析测定植物组织中的吲哚丁酸。
Measurement of indolebutyric Acid in plant tissues by isotope dilution gas chromatography-mass spectrometry analysis.
机构信息
Department of Pomology, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1992 Aug;99(4):1719-22. doi: 10.1104/pp.99.4.1719.
Abstract
An internal standard, [(13)C][indole-2]-indole-3-butyric acid, was synthesized from indole-2[(13)C] and was shown to be effective for the quantitative determination of indole-3-butyric acid from plant tissue. When this standard was used along with [(13)C(6)]indole-3-acetic acid, both indolic auxins could be quantified from the same tobacco (Nicotiana tabacam) leaf sample by isotope dilution analysis using selected ion monitoring gas chromatography-mass spectrometry for detection.
摘要
内标物[(13)C][吲哚-2]-吲哚-3-丁酸由吲哚-2[(13)C]合成，可有效定量测定植物组织中的吲哚-3-丁酸。当与[(13)C(6)]吲哚-3-乙酸一起使用时，通过选择离子监测气相色谱-质谱法检测，同位素稀释分析可从同一烟草（Nicotiana tabacam）叶片样品中同时定量测定这两种吲哚类植物生长素。
相似文献
1
Measurement of indolebutyric Acid in plant tissues by isotope dilution gas chromatography-mass spectrometry analysis.利用同位素稀释气相色谱-质谱分析测定植物组织中的吲哚丁酸。
Plant Physiol. 1992 Aug;99(4):1719-22. doi: 10.1104/pp.99.4.1719.
2
Gas chromatographic analysis of acidic indole auxins in Nicotiana.烟草中酸性吲哚生长素的气相色谱分析
Plant Physiol. 1969 Feb;44(2):267-71. doi: 10.1104/pp.44.2.267.
3
Quantitative determination of indole-3-acetic Acid in sugarbeet leaves using a double standard isotope dilution gas chromatographic assay.采用双标准同位素稀释气相色谱法测定甜菜叶片中的吲哚-3-乙酸。
Plant Physiol. 1986 Jan;80(1):287-90. doi: 10.1104/pp.80.1.287.
4
A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue.一种用于定量分析植物组织中吲哚-3-乙酸和其他生长素的高通量方法。
Anal Biochem. 2008 Jan 15;372(2):177-88. doi: 10.1016/j.ab.2007.08.009. Epub 2007 Aug 11.
5
A Microscale Technique for Gas Chromatography-Mass Spectrometry Measurements of Picogram Amounts of Indole-3-Acetic Acid in Plant Tissues.一种用于植物组织中皮克级吲哚 - 3 - 乙酸气相色谱 - 质谱测量的微尺度技术。
Plant Physiol. 1995 Jul;108(3):1043-1047. doi: 10.1104/pp.108.3.1043.
6
Wounding Nicotiana tabacum Leaves Causes a Decline in Endogenous Indole-3-Acetic Acid.创伤烟草叶片会导致内源吲哚-3-乙酸的减少。
Plant Physiol. 1991 Jul;96(3):802-5. doi: 10.1104/pp.96.3.802.
7
Identification and quantification of three active auxins in different tissues of Tropaeolum majus.金莲花不同组织中三种活性生长素的鉴定与定量分析。
Physiol Plant. 2002 Jun;115(2):320-329. doi: 10.1034/j.1399-3054.2002.1150220.x.
8
9
Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry.高效液相色谱-串联质谱法快速灵敏检测植物样品中的植物激素和类黄酮。
J Sep Sci. 2012 Oct;35(19):2559-66. doi: 10.1002/jssc.201200418. Epub 2012 Jul 19.
10
C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.C(6)-[苯环]-吲哚-3-乙酸：植物中吲哚-3-乙酸定量质谱分析的新内标。
Plant Physiol. 1986 Jan;80(1):14-9. doi: 10.1104/pp.80.1.14.
引用本文的文献
1
Potassium indole-3-butyric acid affects rice's adaptability to salt stress by regulating carbon metabolism, transcription factor genes expression, and biosynthesis of secondary metabolites.吲哚-3-丁酸钾通过调节碳代谢、转录因子基因表达和次生代谢产物的生物合成来影响水稻对盐胁迫的适应性。
Front Plant Sci. 2024 Sep 3;15:1416936. doi: 10.3389/fpls.2024.1416936. eCollection 2024.
2
The NRT1/PTR FAMILY protein NPF7.3/NRT1.5 is an indole-3-butyric acid transporter involved in root gravitropism.NRT1/PTR 家族蛋白 NPF7.3/NRT1.5 是一种吲哚-3-丁酸转运体，参与根的向地性。
Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31500-31509. doi: 10.1073/pnas.2013305117. Epub 2020 Nov 20.
3
Indole 3-Butyric Acid Metabolism and Transport in .吲哚-3-丁酸在……中的代谢与转运
Front Plant Sci. 2019 Jul 3;10:851. doi: 10.3389/fpls.2019.00851. eCollection 2019.
4
Roles for IBA-derived auxin in plant development.IBA 衍生生长素在植物发育中的作用。
J Exp Bot. 2018 Jan 4;69(2):169-177. doi: 10.1093/jxb/erx298.
5
Auxin biosynthesis and storage forms.生长素的生物合成和储存形式。
J Exp Bot. 2013 Jun;64(9):2541-55. doi: 10.1093/jxb/ert080. Epub 2013 Apr 11.
6
Transport of indole-3-butyric acid and indole-3-acetic acid in Arabidopsis hypocotyls using stable isotope labeling.利用稳定同位素标记研究拟南芥下胚轴中吲哚-3-丁酸和吲哚-3-乙酸的运输。
Plant Physiol. 2012 Apr;158(4):1988-2000. doi: 10.1104/pp.111.191288. Epub 2012 Feb 9.
7
Conversion of endogenous indole-3-butyric acid to indole-3-acetic acid drives cell expansion in Arabidopsis seedlings.内源吲哚-3-丁酸向吲哚-3-乙酸的转化驱动拟南芥幼苗细胞的扩展。
Plant Physiol. 2010 Aug;153(4):1577-86. doi: 10.1104/pp.110.157461. Epub 2010 Jun 18.
8
A novel auxin conjugate hydrolase from wheat with substrate specificity for longer side-chain auxin amide conjugates.一种来自小麦的新型生长素共轭水解酶，对较长侧链生长素酰胺共轭物具有底物特异性。
Plant Physiol. 2004 Aug;135(4):2230-40. doi: 10.1104/pp.104.043398. Epub 2004 Aug 6.
9
The rib1 mutant is resistant to indole-3-butyric acid, an endogenous auxin in Arabidopsis.rib1突变体对吲哚-3-丁酸具有抗性，吲哚-3-丁酸是拟南芥中的一种内源生长素。
Plant Physiol. 2000 Dec;124(4):1739-51. doi: 10.1104/pp.124.4.1739.
10
Genetic analysis of indole-3-butyric acid responses in Arabidopsis thaliana reveals four mutant classes.拟南芥中吲哚 - 3 - 丁酸反应的遗传分析揭示了四个突变体类别。
Genetics. 2000 Nov;156(3):1323-37. doi: 10.1093/genetics/156.3.1323.
本文引用的文献
1
Effect of Exogenous Indole-3-Acetic Acid and Indole-3-Butyric Acid on Internal Levels of the Respective Auxins and Their Conjugation with Aspartic Acid during Adventitious Root Formation in Pea Cuttings.外源吲哚-3-乙酸和吲哚-3-丁酸对豌豆插条不定根形成过程中各自生长素的内源水平及其与天冬氨酸结合的影响
Plant Physiol. 1991 Jul;96(3):856-61. doi: 10.1104/pp.96.3.856.
2
Relationship between Indole-3-Acetic Acid Levels in Apple (Malus pumila Mill) Rootstocks Cultured in Vitro and Adventitious Root Formation in the Presence of Indole-3-Butyric Acid.体外培养的苹果（苹果属苹果）砧木中吲哚-3-乙酸水平与吲哚-3-丁酸存在下不定根形成的关系
Plant Physiol. 1989 Feb;89(2):439-43. doi: 10.1104/pp.89.2.439.
3
C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.C(6)-[苯环]-吲哚-3-乙酸：植物中吲哚-3-乙酸定量质谱分析的新内标。
Plant Physiol. 1986 Jan;80(1):14-9. doi: 10.1104/pp.80.1.14.
4
Double-standard isotope dilution assay. I. Quantitative assay of indole-3-acetic acid.双标准同位素稀释分析法。I. 吲哚-3-乙酸的定量分析。
Anal Biochem. 1981 Apr;112(2):249-57. doi: 10.1016/0003-2697(81)90290-6.
Zotero 插件