酵母Gal4：重新审视的转录范例。
Yeast Gal4: a transcriptional paradigm revisited.
作者信息
Traven Ana, Jelicic Branka, Sopta Mary
机构信息
St Vincent's Institute, 9 Princes Street, Fitzroy, Vic 3065, Australia.
出版信息
EMBO Rep. 2006 May;7(5):496-9. doi: 10.1038/sj.embor.7400679.
Abstract
During the past two decades, the yeast Gal4 protein has been used as a model for studying transcriptional activation in eukaryotes. Many of the properties of transcriptional regulation first demonstrated for Gal4 have since been shown to be reiterated in the function of several other eukaryotic transcriptional regulators. Technological advances based on the transcriptional properties of this factor--such as the two-hybrid technology and Gal4-inducible systems for controlled gene expression--have had far-reaching influences in fields beyond transcription. In this review, we provide an updated account of Gal4 function, including data from new technologies that have been recently applied to the study of the GAL network.
摘要
在过去二十年中，酵母Gal4蛋白一直被用作研究真核生物转录激活的模型。最初在Gal4上证明的许多转录调控特性，后来在其他几种真核生物转录调节因子的功能中也得到了体现。基于该因子转录特性的技术进步，如双杂交技术和用于可控基因表达的Gal4诱导系统，在转录以外的领域产生了深远影响。在本综述中，我们提供了Gal4功能的最新情况，包括最近应用于GAL网络研究的新技术的数据。
相似文献
1
Yeast Gal4: a transcriptional paradigm revisited.酵母Gal4：重新审视的转录范例。
EMBO Rep. 2006 May;7(5):496-9. doi: 10.1038/sj.embor.7400679.
2
TORC1 signaling modulates Cdk8-dependent GAL gene expression in Saccharomyces cerevisiae.TORC1 信号调节酿酒酵母中 Cdk8 依赖性 GAL 基因的表达。
Genetics. 2021 Dec 10;219(4). doi: 10.1093/genetics/iyab168.
3
MIG1-dependent and MIG1-independent regulation of GAL gene expression in Saccharomyces cerevisiae: role of Imp2p.酿酒酵母中GAL基因表达的MIG1依赖性和非MIG1依赖性调控：Imp2p的作用
Yeast. 2003 Oct 15;20(13):1085-96. doi: 10.1002/yea.1025.
4
An unexpected role for ubiquitylation of a transcriptional activator.转录激活因子泛素化的意外作用。
Cell. 2005 Mar 25;120(6):733-4. doi: 10.1016/j.cell.2005.03.004.
5
Gene activation by dissociation of an inhibitor from a transcriptional activation domain.通过抑制剂从转录激活结构域解离实现基因激活。
Mol Cell Biol. 2009 Oct;29(20):5604-10. doi: 10.1128/MCB.00632-09. Epub 2009 Aug 3.
6
The DNA-binding domain of yeast Hsf1 regulates both DNA-binding and transcriptional activities.酵母热休克转录因子1（Hsf1）的DNA结合结构域可调控DNA结合及转录活性。
Biochem Biophys Res Commun. 2006 Aug 11;346(4):1324-9. doi: 10.1016/j.bbrc.2006.06.057. Epub 2006 Jun 19.
7
Interdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4p.转录激活因子Gcn4p对SAGA和Srb中介体的相互依赖募集。
Mol Cell Biol. 2005 May;25(9):3461-74. doi: 10.1128/MCB.25.9.3461-3474.2005.
8
Reconstructing genetic networks in yeast.重构酵母中的基因网络。
Nat Biotechnol. 2003 Nov;21(11):1295-7. doi: 10.1038/nbt1103-1295.
9
Epigenetics of the yeast galactose genetic switch.酵母半乳糖遗传开关的表观遗传学。
J Biosci. 2009 Oct;34(4):513-22. doi: 10.1007/s12038-009-0070-y.
10
The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4.酿酒酵母SAGA复合物在体内作为Gal4转录激活的共激活因子发挥作用。
Genes Dev. 2001 Aug 1;15(15):1946-56. doi: 10.1101/gad.911501.
引用本文的文献
1
Divergence in a eukaryotic transcription factor's co-TF dependence involves multiple intrinsically disordered regions.真核转录因子的共转录因子依赖性差异涉及多个内在无序区域。
Nat Commun. 2025 Jun 18;16(1):5340. doi: 10.1038/s41467-025-59244-w.
2
A generalizable approach for programming protease-responsive conformationally inhibited artificial transcriptional factors.一种用于编程蛋白酶响应性构象抑制人工转录因子的通用方法。
Nat Commun. 2025 May 17;16(1):4604. doi: 10.1038/s41467-025-59828-6.
3
Yeast Tools for Studying Type II Topoisomerases in Budding Yeast.用于研究芽殖酵母中II型拓扑异构酶的酵母工具。
Methods Mol Biol. 2025;2928:123-150. doi: 10.1007/978-1-0716-4550-5_12.
4
Animal models in biomedical research: Relevance of .生物医学研究中的动物模型：……的相关性
Heliyon. 2024 Dec 31;11(1):e41605. doi: 10.1016/j.heliyon.2024.e41605. eCollection 2025 Jan 15.
5
Soil microbiome bacteria protect plants against filamentous fungal infections via intercellular contacts.土壤微生物组细菌通过细胞间接触保护植物免受丝状真菌感染。
Proc Natl Acad Sci U S A. 2025 Jan 21;122(3):e2418766122. doi: 10.1073/pnas.2418766122. Epub 2025 Jan 15.
6
Divergence in a Eukaryotic Transcription Factor's co-TF Dependence Involves Multiple Intrinsically Disordered Regions Affecting Activation and Autoinhibition.真核转录因子的共转录因子依赖性差异涉及多个影响激活和自抑制的内在无序区域。
bioRxiv. 2025 Jan 2:2024.04.20.590343. doi: 10.1101/2024.04.20.590343.
7
Unveiling a Microexon Switch: Novel Regulation of the Activities of Sugar Assimilation and Plant-Cell-Wall-Degrading Xylanases and Cellulases by Xlr2 in .揭示微外显子开关：Xlr2 对糖吸收和植物细胞壁降解木聚糖酶和纤维素酶活性的新型调控作用。
Int J Mol Sci. 2024 May 9;25(10):5172. doi: 10.3390/ijms25105172.
8
Reshaping the yeast galactose regulon via GPCR signaling cascade.通过 GPCR 信号级联重塑酵母半乳糖调控网络。
Cell Rep Methods. 2023 Dec 18;3(12):100647. doi: 10.1016/j.crmeth.2023.100647. Epub 2023 Nov 20.
9
Identification of a biosynthetic gene cluster for a red pigment cristazarin produced by a lichen-forming fungus Cladonia metacorallifera.鉴定一种红色色素 cristazarin 的生物合成基因簇，该色素由地衣形成真菌 Cladonia metacorallifera 产生。
PLoS One. 2023 Jun 23;18(6):e0287559. doi: 10.1371/journal.pone.0287559. eCollection 2023.
10
Transcription factor clusters enable target search but do not contribute to target gene activation.转录因子簇可实现靶基因搜索，但不促进靶基因激活。
Nucleic Acids Res. 2023 Jun 23;51(11):5449-5468. doi: 10.1093/nar/gkad227.
本文引用的文献
1
Targets of the Gal4 transcription activator in functional transcription complexes.功能转录复合物中Gal4转录激活因子的靶标。
Mol Cell Biol. 2005 Oct;25(20):9092-102. doi: 10.1128/MCB.25.20.9092-9102.2005.
2
Function of a eukaryotic transcription activator during the transcription cycle.真核转录激活因子在转录周期中的功能。
Mol Cell. 2005 Apr 29;18(3):369-78. doi: 10.1016/j.molcel.2005.03.029.
3
The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing.F 盒蛋白 Dsg1/Mdm30 是一种转录共激活因子，可刺激 Gal4 周转和共转录 mRNA 加工。
Cell. 2005 Mar 25;120(6):887-99. doi: 10.1016/j.cell.2004.12.025.
4
The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription.酿酒酵母Srb8 - Srb11复合物在Gal4激活的转录过程中与SAGA复合物协同发挥作用。
Mol Cell Biol. 2005 Jan;25(1):114-23. doi: 10.1128/MCB.25.1.114-123.2005.
5
Targeting of Swi/Snf to the yeast GAL1 UAS G requires the Mediator, TAF IIs, and RNA polymerase II.Swi/Snf靶向酵母GAL1上游激活序列（UASG）需要中介体、TATA结合蛋白相关因子（TAF IIs）和RNA聚合酶II。
EMBO J. 2004 Oct 13;23(20):4040-50. doi: 10.1038/sj.emboj.7600416. Epub 2004 Sep 23.
6
In vivo target of a transcriptional activator revealed by fluorescence resonance energy transfer.通过荧光共振能量转移揭示转录激活因子的体内靶点。
Genes Dev. 2004 Feb 1;18(3):333-43. doi: 10.1101/gad.1148404.
7
Association of the Mediator complex with enhancers of active genes.中介体复合物与活跃基因增强子的关联。
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13887-91. doi: 10.1073/pnas.2036346100. Epub 2003 Nov 17.
8
Independent recruitment in vivo by Gal4 of two complexes required for transcription.Gal4在体内独立募集转录所需的两种复合物。
Mol Cell. 2003 May;11(5):1301-9. doi: 10.1016/s1097-2765(03)00144-8.
9
Transcriptional activating regions target a cyclin-dependent kinase.转录激活区域靶向一种细胞周期蛋白依赖性激酶。
Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14706-9. doi: 10.1073/pnas.232573899. Epub 2002 Nov 4.
10
Differential requirement of SAGA components for recruitment of TATA-box-binding protein to promoters in vivo.体内SAGA组分在将TATA盒结合蛋白募集至启动子过程中的差异需求。
Mol Cell Biol. 2002 Nov;22(21):7365-71. doi: 10.1128/MCB.22.21.7365-7371.2002.
Zotero 插件