Kabata J, Gratwohl A, Tichelli A, John L, Speck B
Department of Research, Kantonsspital Basel, Switzerland.
Lab Anim Sci. 1991 Dec;41(6):613-9.
Hematologic values of peripheral blood from normal adult New Zealand White rabbits were determined by five different automated flow cytometers in use in a routine clinical hematology laboratory: Technicon H1, Coulter Counter CC540, Coulter Counter VCS, Sysmex NE8000 and Sysmex R1000. The software designed for human blood analysis was used in all instances without adaptation. The total numbers of white blood cells, red blood cells, reticulocytes and platelets were measured with high precision and accuracy. Except for hemoglobin content, concordance was excellent for all measured and calculated values among the different automated flow cytometers. Determining the white blood cell differential count was more complex. Eosinophils and lymphocytes were quantified reliably by all the automated flow cytometers used. However, the results were rejected by Technicon H1 and Sysmex NE8000 in 50% of the cases. Rabbit basophils were recognized with accuracy by Technicon H1 only. The proportion of polymorphonuclear versus mononuclear white cells was identical when measured with Technicon H1 and Coulter Counter VCS. These results show that the new generation of automated flow cytometers designed for human blood can be used with some limitations for animal studies. They allow the standardization of normal values and comparison of results among or between laboratories. They also introduce new parameters, the value of which is as yet undefined.
在一个常规临床血液学实验室中，使用了五台不同的自动流式细胞仪来测定正常成年新西兰白兔外周血的血液学值，这些仪器分别是：Technicon H1、库尔特计数器CC540、库尔特计数器VCS、Sysmex NE8000和Sysmex R1000。所有情况下均使用专为人类血液分析设计的软件，未作调整。白细胞、红细胞、网织红细胞和血小板的总数测量具有高精度和准确性。除血红蛋白含量外，不同自动流式细胞仪之间所有测量和计算值的一致性都非常好。确定白细胞分类计数更为复杂。所有使用的自动流式细胞仪都能可靠地定量嗜酸性粒细胞和淋巴细胞。然而，Technicon H1和Sysmex NE8000在50%的病例中结果被拒收。只有Technicon H1能准确识别兔嗜碱性粒细胞。使用Technicon H1和库尔特计数器VCS测量时，多形核白细胞与单核白细胞的比例相同。这些结果表明，为人类血液设计的新一代自动流式细胞仪在动物研究中使用时存在一些局限性。它们允许标准化正常值并在不同实验室之间比较结果。它们还引入了一些新参数，其价值尚未确定。
