Thone-Reineke C, Kalk P, Dorn M, Klaus S, Simon K, Pfab T, Godes M, Persson P, Unger T, Hocher B
Center for Cardiovascular Research, Department of Pharmacology and Toxicology, Charité-Universitätmedizin, Berlin.
Am J Physiol Regul Integr Comp Physiol. 2006 Oct;291(4):R1025-30. doi: 10.1152/ajpregu.00898.2005. Epub 2006 May 4.
Maternal low-protein diet during pregnancy is a risk factor for cardiovascular disease of the offspring in later life. The impact of high-protein diet during pregnancy on the cardiovascular phenotype of the offspring, however, is still unknown. We examined the influence of a high-protein diet during pregnancy and lactation on the renal, hemodynamic, and metabolic phenotype of the F1 generation. Female Wistar rats were either fed a normal protein diet (20% protein: NP) or an isocaloric high-protein diet (40% protein: HP) throughout pregnancy and lactation. At weaning, the offspring were fed with standard diet, and they were allocated according to sex and maternal diet to four groups: normal-protein male (NPm, n = 25), normal-protein female (NPf, n = 19), high-protein male (HPm, n = 24), high-protein female (HPf, n = 29). During the experiment (22 wk), the animals were characterized by repeated measurement of body weight, food intake, blood pressure, glucose tolerance, energy expenditure, and kidney function. At the end of the study period histomorphological analyses of the kidneys and weight measurement of reproductive fat pads were conducted. There were no differences in birth weight between the study groups. No influence of maternal diet on energy expenditure, glucose tolerance, and plasma lipid levels was detected. Blood pressure and glomerulosclerosis were elevated in male offspring only, whereas female offspring were characterized by an increased food efficiency, higher body weight, and increased fat pads. Our study demonstrates that a high-protein diet during pregnancy and lactation in rats programs blood pressure, food efficiency, and body weight of the offspring in a sex-dependent manner.
孕期母体低蛋白饮食是后代日后患心血管疾病的一个风险因素。然而，孕期高蛋白饮食对后代心血管表型的影响仍不清楚。我们研究了孕期和哺乳期高蛋白饮食对F1代肾脏、血流动力学和代谢表型的影响。雌性Wistar大鼠在整个孕期和哺乳期要么喂食正常蛋白饮食（20%蛋白质：NP），要么喂食等热量高蛋白饮食（40%蛋白质：HP）。断奶时，后代喂食标准饮食，并根据性别和母体饮食分为四组：正常蛋白雄性（NPm，n = 25）、正常蛋白雌性（NPf，n = 19）、高蛋白雄性（HPm，n = 24）、高蛋白雌性（HPf，n = 29）。在实验期间（22周），通过重复测量体重、食物摄入量、血压、葡萄糖耐量、能量消耗和肾功能来对动物进行表征。在研究期结束时，对肾脏进行组织形态学分析并测量生殖脂肪垫的重量。研究组之间出生体重没有差异。未检测到母体饮食对能量消耗、葡萄糖耐量和血浆脂质水平有影响。仅雄性后代的血压和肾小球硬化有所升高，而雌性后代的特征是食物效率提高、体重增加和脂肪垫增多。我们的研究表明，大鼠孕期和哺乳期的高蛋白饮食会以性别依赖的方式影响后代的血压、食物效率和体重。
