Fleire S J, Goldman J P, Carrasco Y R, Weber M, Bray D, Batista F D
Lymphocyte Interaction Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK.
Science. 2006 May 5;312(5774):738-41. doi: 10.1126/science.1123940.
B cells recognize foreign antigens by virtue of cell surface immunoglobulin receptors and are most effectively activated by membrane-bound ligands. Here, we show that in the early stages of this process, B cells exhibit a two-phase response in which they first spread over the antigen-bearing membrane and then contract, thereby collecting bound antigen into a central aggregate. The extent of this response, which is both signaling- and actin-dependent, determines the quantity of antigen accumulated and hence the degree of B cell activation. Brownian dynamic simulations reproduce essential features of the antigen collection process and suggest a possible basis for affinity discrimination. We propose that dynamic spreading is an important step of the immune response.
B细胞凭借细胞表面免疫球蛋白受体识别外来抗原，并且最有效地被膜结合配体激活。在此，我们表明在这一过程的早期阶段，B细胞呈现出两阶段反应，即它们首先在承载抗原的膜上铺展，然后收缩，从而将结合的抗原收集到一个中央聚集体中。这种反应的程度依赖于信号传导和肌动蛋白，它决定了积累的抗原量，进而决定了B细胞的激活程度。布朗动力学模拟再现了抗原收集过程的基本特征，并提出了亲和力识别的可能基础。我们认为动态铺展是免疫反应的一个重要步骤。
