Fraysse Benoit, Mons Raphael, Garric Jeanne
Laboratoire d'Ecotoxicologie, Cemagref 3 bis quai Chauveau, CP 220, 69336 Lyon Cedex 09, France.
Ecotoxicol Environ Saf. 2006 Feb;63(2):253-67. doi: 10.1016/j.ecoenv.2004.10.015.
A 4-day embryo-larval zebrafish test, from blastula stage to hatching included, was developed. The observations of embryo developmental were made at different development stages, for which morphological, physiological, and behavioral endpoints were selected and quantified for unexposed and exposed embryos. The sensitivity and the ability of these endpoints to inform about mode of action (MoA) were established in testing three model toxicants with well-known toxic effects (propranolol, malathion, cadmium). Lethal, sublethal (heart rate/edema, spontaneous movements, and hatching rate/time disturbance), and teratogenic effects were detected for all the studied compounds. This bioassay allows characterization of impairments at different biological levels: neuromuscular, physiological, morphological, and behavioral, and brings useful information about the toxic MoA of the chemicals on nontarget organisms. In this sense to answers the chemical industries and international organization (EMEA) requirements for the environmental risk assessment of new chemicals and pharmaceuticals.
开发了一种为期4天的斑马鱼胚胎-幼体试验，涵盖从囊胚期到孵化阶段。在不同发育阶段对胚胎发育进行观察，针对未暴露和暴露的胚胎选择并量化形态、生理和行为终点。通过测试三种具有已知毒性效应的模型毒物（普萘洛尔、马拉硫磷、镉），确定了这些终点对作用模式（MoA）的敏感性和告知能力。对所有研究化合物均检测到致死、亚致死（心率/水肿、自发运动和孵化率/时间紊乱）和致畸效应。这种生物测定法能够在不同生物水平（神经肌肉、生理、形态和行为）上表征损伤情况，并为化学品对非靶标生物的毒性作用模式提供有用信息。从这个意义上讲，它满足了化学工业和国际组织（欧洲药品管理局）对新化学品和药品环境风险评估的要求。
