Wu Hao, Guo Hongwei, Zhao Rui
Department of Nutrition & Food Hygiene, School of Public Health, Fudan University, Shanghai, P. R. China.
Yakugaku Zasshi. 2006 May;126(5):365-71. doi: 10.1248/yakushi.126.365.
The effects of polysaccharide extracted from Lycium barbarum (LBP) on blood glucose, oxidative stress and DNA damage in rats with non-insulin dependent diabetes mellitus (NIDDM) were studied. The results show that LBP treatment (10 mg/kg.d) for 4 weeks led to decreased levels of blood glucose, malondialdehyde (MDA) and nitric oxide (NO) in serum of fasting rats; and to increased serum level of superoxidedismutase (SOD). Furthermore, LBP could reduce cellular DNA damage in peripheral lymphocytes of NIDDM rats. The DNA damage was determined by using the single cell gel (comet) assay with alkaline electrophoresis and was quantified by measuring tail length and tail moment. These results suggest that LBP can control blood glucose and modulate the metabolism of glucose, leading to significant improvement of oxidative stress markers (SOD, MDA) in rats with NIDDM. And that, LBP decreases DNA damage possibly via a decrease in oxidative stress levels. In conclusion, LBP as a dietary supplement may prevent the development of complications or even tendency to carcinogenesis in NIDDM rats.
研究了枸杞多糖（LBP）对非胰岛素依赖型糖尿病（NIDDM）大鼠血糖、氧化应激及DNA损伤的影响。结果显示，连续4周给予LBP（10毫克/千克·天）可使空腹大鼠血清中的血糖、丙二醛（MDA）和一氧化氮（NO）水平降低；超氧化物歧化酶（SOD）血清水平升高。此外，LBP可减轻NIDDM大鼠外周淋巴细胞的细胞DNA损伤。采用碱性电泳单细胞凝胶（彗星）试验测定DNA损伤，并通过测量尾长和尾矩进行定量分析。这些结果表明，LBP可控制血糖并调节葡萄糖代谢，使NIDDM大鼠的氧化应激标志物（SOD、MDA）显著改善。而且，LBP可能通过降低氧化应激水平来减少DNA损伤。总之，LBP作为一种膳食补充剂可能预防NIDDM大鼠并发症的发生，甚至预防致癌倾向。
