Wang Wei, Liu Mingxing, Wang Yang, Yang Tao, Li Dongsheng, Ding Feng, Sun Hongzhi, Bai Guang, Li Qing
Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, China.
Department of Internal Medicine, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, China.
Evid Based Complement Alternat Med. 2018 Jan 28;2018:2305683. doi: 10.1155/2018/2305683. eCollection 2018.
polysaccharide (LBP) is the major function component of L. and has been previously reported to induce the phenotypic and functional maturation of dendritic cells (DCs) as well as activating T lymphocytes. In the current study, the immunologic cytotoxicity promoting effect of LBP was assessed and the underlying mechanism was explored. The impact of LBP on the phenotype, maturation, and immunogenicity of DCs was assessed. The activity of Notch pathway which is involved in the regulation of LBP on DCs was detected. Afterwards, the influence of LBP on cytotoxicity of DC-mediated cytotoxicity T lymphocytes (CTLs) to CT26-WT colon cancer cells was further assessed. Administration of LBP induced the phenotypic and functional maturation of DCs. After being subjected to LBP, the expression of Notch and Jagged and Notch targets Hes1 and Hes5 was all upregulated. The cytotoxicity of DC-mediated CTLs was strengthened by administration of LBP. Additionally, cytotoxicity of DC-mediated CTLs on CT26-WT colon cancer cells also increased with effector-target ratio. In conclusion, LBP could induce the phenotypic and functional maturation of DCs via Notch signaling and promote the cytotoxicity of DC-mediated CTLs, which could be employed as a promising adjuvant for cancer immunotherapy.
枸杞多糖（LBP）是枸杞的主要功能成分，此前已有报道称其可诱导树突状细胞（DCs）的表型和功能成熟以及激活T淋巴细胞。在本研究中，评估了LBP的免疫细胞毒性促进作用并探讨了其潜在机制。评估了LBP对DCs的表型、成熟和免疫原性的影响。检测了参与LBP对DCs调节的Notch信号通路的活性。随后，进一步评估了LBP对DC介导的细胞毒性T淋巴细胞（CTLs）对CT26-WT结肠癌细胞的细胞毒性的影响。给予LBP可诱导DCs的表型和功能成熟。在接受LBP处理后，Notch、Jagged以及Notch靶标Hes1和Hes5的表达均上调。给予LBP可增强DC介导的CTLs的细胞毒性。此外，DC介导的CTLs对CT26-WT结肠癌细胞的细胞毒性也随着效应细胞与靶细胞比例的增加而增强。总之，LBP可通过Notch信号通路诱导DCs的表型和功能成熟，并促进DC介导的CTLs的细胞毒性，有望作为癌症免疫治疗的佐剂。
