Varoni Maria Vittoria, Gadau Sergio Domenico, Pasciu Valeria, Baralla Elena, Serra Elisa, Palomba Domenico, Demontis Maria Piera
Department of Veterinary Medicine, University of Sassari, Sassari, Italy.
Department of Veterinary Medicine, University of Sassari, Sassari, Italy.
Exp Mol Pathol. 2017 Aug;103(1):26-32. doi: 10.1016/j.yexmp.2017.06.003. Epub 2017 Jun 20.
This study describes the effects of Lycium barbarum polysaccharides (LBP) on testicular damage induced by cadmium (Cd). Adult male rats were i.p. injected with CdCl (4mg/Kg, once) with or without LBP pretreatment (300mg/Kg orally, once a day, for 30days). Testis weight, morphological/histological structure and oxidative stress parameters were evaluated. Several adverse effects were observed after CdCl injection, with a significant decrease in body/testis weight ratio (P<0.05), gross morphological changes with hyperemia of the parenchyma, increased volume and alteration in the structure of the seminiferous tubules. Furthermore, Cd intoxication caused a significant decrease of glutathione (GSH) and Trolox equivalent antioxidant capacity (TEAC) in testis (P<0.05) together with a significant increase (P<0.01) of 3-nitro-l-tyrosine (3NT) while malondialdehyde (MDA) did not change. LBP pretreatment caused slight signs of improvement in the morphology of the seminiferous tubules. Our results confirm that Cd induces testicular damage and suggest the oxidative stress involvement. LBP could ameliorate Cd testicular damage but further investigations are needed.
本研究描述了枸杞多糖（LBP）对镉（Cd）诱导的睾丸损伤的影响。成年雄性大鼠腹腔注射CdCl（4mg/Kg，一次），同时或不进行LBP预处理（口服300mg/Kg，每天一次，共30天）。评估睾丸重量、形态学/组织学结构和氧化应激参数。注射CdCl后观察到几种不良反应，体/睾重量比显著降低（P<0.05），大体形态改变，实质充血，曲细精管体积增大和结构改变。此外，镉中毒导致睾丸中谷胱甘肽（GSH）和Trolox等效抗氧化能力（TEAC）显著降低（P<0.05），同时3-硝基-L-酪氨酸（3NT）显著增加（P<0.01），而丙二醛（MDA）没有变化。LBP预处理使曲细精管形态略有改善迹象。我们的结果证实镉会诱导睾丸损伤，并提示氧化应激参与其中。LBP可以改善镉引起的睾丸损伤，但还需要进一步研究。
