Delisse A M, Descurieux M, Rutgers T, D'Hondt E, De Wilde M, Arima T, Barrera-Sala J M, Ercilla M G, Ruelle J L, Cabezon T
SmithKline Beecham Biologicals, Rixensart, Belgium.
J Hepatol. 1991;13 Suppl 4:S20-3. doi: 10.1016/0168-8278(91)90017-6.
cDNA fragments encoding the putative structural genes of the hepatitis C genome were isolated from a plasma pool of Japanese non-A, non-B hepatitis patients and from sera of individual Spanish patients. From the Japanese plasma pool a series of E1 clones was obtained that showed 88-98% homology among each other, both at the nucleotide and amino acid level. Compared to the sequences published by the Chiron Corporation and Takeuchi et al., the amino acid homology was 75-79% and 91-94%, respectively. Analysis of the core and E2/NS1 genes showed a high conservation of the core sequence and a high sequence variation in the 5' end of the E2/NS1 gene. The E1 gene of one Spanish isolate showed greater homology to the Chiron than to the Japanese sequence. Another Spanish isolate was more homologous to the Japanese sequence indicating that both hepatitis C genotypes are present in Europe. Analysis of the E1 gene of an isolate derived from a single patient with a 5-year interval revealed nine nucleotide and five amino acid changes.
从日本非甲非乙型肝炎患者的血浆池以及西班牙个体患者的血清中分离出编码丙型肝炎病毒基因组推定结构基因的cDNA片段。从日本血浆池中获得了一系列E1克隆，这些克隆在核苷酸和氨基酸水平上彼此间显示出88%-98%的同源性。与Chiron公司和竹内等人公布的序列相比，氨基酸同源性分别为75%-79%和91%-94%。核心基因和E2/NS1基因分析表明，核心序列高度保守，而E2/NS1基因5'端序列变异较大。一株西班牙分离株的E1基因与Chiron公司的序列比对，比对日本序列显示出更高的同源性。另一株西班牙分离株与日本序列的同源性更高，这表明欧洲同时存在两种丙型肝炎病毒基因型。对一名间隔5年的患者分离株的E1基因分析发现了9个核苷酸变化和5个氨基酸变化。
