QuantiScientifics LLC, Orange, CA, USA.
Methods Mol Biol. 2023;2612:225-238. doi: 10.1007/978-1-0716-2903-1_16.
Enzyme-linked immunosorbent assay (ELISA) is by definition a biosensor. However, not all immuno-biosensors involve the use of enzymes, while other biosensors incorporate ELISA as a key signaling component. In this chapter, we review the role of ELISA in signal amplification, integration with microfluidic systems, digital labeling, and electrochemical detection.
酶联免疫吸附测定（ELISA）从定义上讲就是一种生物传感器。然而，并非所有免疫生物传感器都涉及酶的使用，而其他生物传感器则将 ELISA 作为关键信号组件纳入其中。在本章中，我们回顾了 ELISA 在信号放大、与微流控系统集成、数字标记和电化学生物传感器检测中的作用。
