Gilman-Sachs A, Lubinski J, Beer A E, Brend S, Beaman K D
Department of Microbiology and Immunology, University of Health Sciences, Chicago Medical School, IL 60064.
J Clin Lab Immunol. 1991 Jun;35(2):83-8.
To determine the importance of assaying for anti-phospholipid (PL) antibodies other than anti-cardiolipin antibodies in patients with putative anti-phospholipid antibody syndrome (APA), 1,513 serum samples from over 399 patients with the potential for thromboembolic disease were tested for IgM, IgG or IgA class antibodies to the following phospholipids: cardiolipin, phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidic acid. Non-specific binding was subtracted from total binding of antibodies to each PL to eliminate false positive ELISA results. Only patients whose sera contained antibody levels which were greater than three standard deviations above the means were considered positive. Patients who were positive for anti-PL antibody had antibodies to at least one of six phospholipids tested. Approximately 60% of the positive samples contained antibodies to a phospholipid other than cardiolipin. For IgG class, antibodies to phosphatidylserine were the most prevalent followed by antibodies to phosphatidylethanolamine and cardiolipin. These findings indicate that anti-phospholipid antibody analysis should be performed for more than anti-cardiolipin antibody to detect patients with APA.
为了确定在疑似抗磷脂抗体综合征（APA）患者中检测除抗心磷脂抗体之外的抗磷脂（PL）抗体的重要性，对来自399例以上有血栓栓塞性疾病潜在风险患者的1513份血清样本进行了检测，以查找针对以下磷脂的IgM、IgG或IgA类抗体：心磷脂、磷脂酰丝氨酸、磷脂酰肌醇、磷脂酰乙醇胺、磷脂酰甘油和磷脂酸。从抗体与每种PL的总结合中减去非特异性结合，以消除酶联免疫吸附测定（ELISA）的假阳性结果。仅将血清中抗体水平高于平均值三个标准差以上的患者视为阳性。抗PL抗体阳性的患者对所检测的六种磷脂中的至少一种有抗体。大约60%的阳性样本含有除心磷脂之外的磷脂抗体。对于IgG类，磷脂酰丝氨酸抗体最为常见，其次是磷脂酰乙醇胺抗体和心磷脂抗体。这些发现表明，抗磷脂抗体分析不应仅检测抗心磷脂抗体，还应检测其他抗体，以便发现APA患者。
