Sunday M E, Choi N, Spindel E R, Chin W W, Mark E J
Department of Pathology, Harvard Medical School, Boston, MA.
Hum Pathol. 1991 Oct;22(10):1030-9. doi: 10.1016/0046-8177(91)90011-d.
Gastrin-releasing peptide (GRP; mammalian bombesin) is present in the neuroendocrine cells of human fetal lung and in small cell lung carcinomas (SCLCs), where it may act as a growth factor. Considering the potential importance of GRP as a tumor marker, we have conducted a retrospective immunohistochemical analysis of 176 lung tumors for markers of GRP gene expression, as well as several other markers of neuroendocrine cell differentiation: chromogranin A, neuron-specific enolase, and calcitonin. The majority of carcinoids contained mature GRP, in contrast to only a minority of SCLCs and large cell lung carcinomas (LCLCs). However, a majority of SCLCs and LCLCs contained proGRP immunoreactivity. In situ hybridization did not add any information beyond what was obtained using proGRP antisera. In spite of sharing these neuroendocrine cell markers, SCLCs are associated with a graver prognosis than LCLCs. No prognostic significance was associated with immunostaining for GRP or several other markers of neuroendocrine cell differentiation.
胃泌素释放肽（GRP；哺乳动物铃蟾肽）存在于人类胎儿肺的神经内分泌细胞以及小细胞肺癌（SCLC）中，在这些部位它可能作为一种生长因子发挥作用。鉴于GRP作为肿瘤标志物的潜在重要性，我们对176例肺肿瘤进行了回顾性免疫组织化学分析，检测GRP基因表达标志物以及神经内分泌细胞分化的其他几种标志物：嗜铬粒蛋白A、神经元特异性烯醇化酶和降钙素。大多数类癌含有成熟的GRP，相比之下，只有少数小细胞肺癌和大细胞肺癌（LCLC）含有。然而，大多数小细胞肺癌和大细胞肺癌含有前胃泌素释放肽免疫反应性。原位杂交并未提供超出使用前胃泌素释放肽抗血清所获得的任何信息。尽管共享这些神经内分泌细胞标志物，但小细胞肺癌的预后比大细胞肺癌更严重。GRP免疫染色或神经内分泌细胞分化的其他几种标志物与预后无关。
