Chiumiento A, Dominguez A, Lamponi S, Villalonga R, Barbucci R
CRISMA and Department of Chemical and Biosystem Sciences and Tecnologies, University of Siena, Via A. Moro n. 2, Siena, 53100, Italy.
J Mater Sci Mater Med. 2006 May;17(5):427-35. doi: 10.1007/s10856-006-8470-0.
Superoxide dismutase (SOD) was chemically bound to carboxymethyl-cellulose (CMC) polymer.Furthermore, SOD was also trapped into two hydrogels of CMC with 50% and 90% crosslinking degree. The ability of the two SOD-CMC hydrogels to capture SOD and their release kinetics were investigated. ATR FT-IR spectrometry was used to study the conformation of SOD interacting with both CMC polymer and hydrogels. The effect of SOD-CMC polymer conjugate and SOD-CMC hydrogel systems upon human fibroblasts was studied in vitro measuring the cell proliferation inhibition index and evaluating cell morphology. Using the xanthine oxidase-nitroblue tetrazolium assay, the specific activity of bound SOD to CMC polymer or trapped into hydrogels was evaluated. The specific activity of the enzyme was higher in SOD-CMC hydrogels than in SOD-CMC polymer conjugates.
超氧化物歧化酶（SOD）被化学结合到羧甲基纤维素（CMC）聚合物上。此外，SOD还被包埋在交联度分别为50%和90%的两种CMC水凝胶中。研究了这两种SOD-CMC水凝胶捕获SOD的能力及其释放动力学。采用衰减全反射傅里叶变换红外光谱法（ATR FT-IR）研究SOD与CMC聚合物和水凝胶相互作用时的构象。通过体外测量细胞增殖抑制指数并评估细胞形态，研究了SOD-CMC聚合物共轭物和SOD-CMC水凝胶体系对人成纤维细胞的影响。使用黄嘌呤氧化酶-硝基蓝四唑法评估结合到CMC聚合物上或包埋在水凝胶中的SOD的比活性。酶的比活性在SOD-CMC水凝胶中高于SOD-CMC聚合物共轭物。
