Barbatis C, Loukas L, Grigoriou M, Nikolaou I, Tsikou-Papafragou A, Marsan N, Gatter K C, Kaklamanis L
Department of Histopathology, Red Cross Hospital, Athens, Greece.
Clin Mol Pathol. 1995 Aug;48(4):M194-7. doi: 10.1136/mp.48.4.m194.
Aim-To investigate the expression of p53 protein in invasive squamous cell carcinoma (SCC) of the larynx and dysplasia in relation to histological grade and tobacco smoking.Method-Paraffin wax embedded tissue sections from 41 cases of invasive SCC of the larynx, 28 cases of dysplasia and 14 control laryngeal biopsy specimens were studied immunohistochemically using two anti-p53 monoclonal antibodies (DO7 and 1801). The Streptavidin/horseradish peroxidase method was used after microwave antigen retrieval and a semiquantitative method was applied to assess the extent of p53 expression.Results-Of the cases of invasive SCC of the larynx, 78% (32/41), regardless of histological grade, overexpressed p53 compared with only 30% (eight of 28) of cases of mild dysplasia. A gradual increase in p53 expression from mild to severe dysplasia (60%) was observed, and only three of 14 control biopsy specimens of laryngeal nodules showed occasional weakly positive basal cells.Conclusion-The gradual increase in p53 expression from mild to severe dysplasia to invasive SCC indicates that p53 overexpression is an early event in laryngeal carcinogenesis which may lead to invasive malignancy. p53 overexpression may be related to environmental factors as most of the patients smoked tobacco. Microwave postfixation may be essential for the reliable detection of p53.
目的——研究p53蛋白在喉浸润性鳞状细胞癌（SCC）和发育异常中的表达与组织学分级及吸烟的关系。方法——采用两种抗p53单克隆抗体（DO7和1801），对41例喉浸润性SCC、28例发育异常以及14例对照喉活检标本的石蜡包埋组织切片进行免疫组织化学研究。在微波抗原修复后采用链霉亲和素/辣根过氧化物酶法，并应用半定量方法评估p53表达程度。结果——喉浸润性SCC病例中，78%（32/41）无论组织学分级如何均p53过表达，而轻度发育异常病例中只有30%（28例中的8例）如此。观察到从轻度到重度发育异常p53表达逐渐增加（60%），并且14例喉结节对照活检标本中只有3例显示偶尔有弱阳性基底细胞。结论——从轻度发育异常到重度发育异常再到浸润性SCC，p53表达逐渐增加，表明p53过表达是喉癌发生中的早期事件，可能导致浸润性恶性肿瘤。p53过表达可能与环境因素有关，因为大多数患者吸烟。微波后固定对于可靠检测p53可能至关重要。
