Bosari S, Roncalli M, Viale G, Bossi P, Coggi G
Second Department of Pathology, University of Milan School of Medicine, Italy.
J Pathol. 1993 Apr;169(4):425-30. doi: 10.1002/path.1711690407.
Immunoreactivity for the tumour suppressor gene product p53 is commonly found in many different human malignancies and few premalignant lesions. Data on cervical neoplasms, however, are still lacking. We retrospectively investigated p53 immunoreactivity in 92 lesions of the uterine cervix, including 44 cases of chronic cervicitis, 29 squamous intraepithelial lesions (SILs), and 19 invasive carcinomas. p53 immunoreactivity, confined to the basal cell layer, was detected in 74 per cent of cases showing chronic cervicitis and in all cases with low-grade SILs. Conversely, suprabasal and/or diffuse p53 immunoreactivity was exclusively demonstrated in 25 per cent of high-grade SILs and in 74 per cent of invasive carcinomas. The results of this investigation document a high prevalence of p53-immunoreactive malignant tumours of the uterine cervix. In high-grade SILs, p53-immunoreactive cells paralleled the height of involvement by dysplastic changes within the squamous epithelium. A prolonged half-life of the protein is the most likely explanation for the occurrence of p53 immunoreactivity in neoplastic cells. The unexpected finding of p53-immunoreactive cells in inflammatory lesions, though possibly related to an increased proliferation rate of the basal cell compartment, requires further study and underlines the need for a careful approach to p53 immunocytochemistry.
肿瘤抑制基因产物p53的免疫反应性常见于多种不同的人类恶性肿瘤中，而在癌前病变中则较少见。然而，关于宫颈肿瘤的数据仍然缺乏。我们回顾性研究了92例子宫颈病变中的p53免疫反应性，包括44例慢性宫颈炎、29例鳞状上皮内病变（SILs）和19例浸润性癌。p53免疫反应性局限于基底细胞层，在74%的慢性宫颈炎病例和所有低级别SIL病例中均有检测到。相反，在25%的高级别SIL和74%的浸润性癌中仅表现为基底上层和/或弥漫性p53免疫反应性。本研究结果表明子宫颈p53免疫反应性恶性肿瘤的发生率很高。在高级别SIL中，p53免疫反应性细胞与鳞状上皮内发育异常改变的累及高度平行。蛋白质半衰期延长是肿瘤细胞中出现p53免疫反应性的最可能解释。炎症病变中出现p53免疫反应性细胞这一意外发现，尽管可能与基底细胞区增殖率增加有关，但仍需要进一步研究，并强调了对p53免疫细胞化学采取谨慎方法的必要性。
