Mondal Debashri, Saha Kaushik, Datta Chhanda, Chatterjee Uttara, Sengupta Arunabho
Theism (NABL accredited) Laboratory, Flat C/8, Govt. Rental Housing Estate, 65/66B Rani Harshamukhi Road, Kolkata, 700002 West Bengal India.
Department of Pathology, IPGME & R, Kolkata, India.
Indian J Otolaryngol Head Neck Surg. 2013 Apr;65(2):126-33. doi: 10.1007/s12070-012-0590-x. Epub 2012 Nov 8.
Precise assessment of the biological behavior and progression of squamous epithelial lesions of the larynx with a view to predict the prognosis and therapeutic challenges remains an elusive goal. The knowledge and data regarding the expression of proliferative markers indicating the biological activity in different histological grades of squamous epithelial lesions are lacking till date. To evaluate the relationship between Ki67, p27 and p53 expression as well as topographic distribution of Ki67 with the histological subtypes or grades of laryngeal squamous intraepithelial and invasive lesions. Sixty-two consecutive cases with histologically documented intraepithelial and invasive squamous lesion were studied for Ki67, p27 and p53 expression. Mann-Whitney U, Kruskal-Wallis and Spearman's correlation tests were used for statistical analysis. The mean Ki67 labeling index in hyperplasia, dysplasia and carcinoma were 12.15, 22.03 and 35.53 % respectively and this difference was statistically significant (P < 0.05). There was strong positive correlation between Ki67 labeling index and increasing grades of squamous lesions. p27 expression was progressively decreased and p53 expression was progressively increased as the lesions progressed from hyperplasia to dysplasia and dysplasia to carcinoma. The topographic distribution of Ki67 positive cells increased with progressive grades of dysplasia. The Ki67 labeling index correlates well with the histological grade of both intraepithelial and invasive lesions of the larynx. And the topographic distribution of Ki67 expression depends on the grade of the dysplasia. Hence, Ki67 expression has a definite role in predicting the biological behavior of the lesions.
精确评估喉鳞状上皮病变的生物学行为和进展情况，以期预测预后和治疗挑战，这仍然是一个难以实现的目标。迄今为止，关于增殖标志物在不同组织学分级的鳞状上皮病变中表达情况以指示生物学活性的知识和数据仍然匮乏。为了评估Ki67、p27和p53的表达之间的关系，以及Ki67的拓扑分布与喉鳞状上皮内病变和浸润性病变的组织学亚型或分级之间的关系。对62例经组织学证实的上皮内和浸润性鳞状病变连续病例进行了Ki67、p27和p53表达的研究。采用曼-惠特尼U检验、克鲁斯卡尔-沃利斯检验和斯皮尔曼相关性检验进行统计分析。增生、发育异常和癌中的平均Ki67标记指数分别为12.15%、22.03%和35.53%，这种差异具有统计学意义（P<0.05）。Ki67标记指数与鳞状病变分级增加之间存在强正相关。随着病变从增生发展为发育异常，再从发育异常发展为癌，p27表达逐渐降低，p53表达逐渐增加。Ki67阳性细胞的拓扑分布随着发育异常程度的增加而增加。Ki67标记指数与喉上皮内病变和浸润性病变的组织学分级密切相关。并且Ki67表达的拓扑分布取决于发育异常的分级。因此，Ki67表达在预测病变的生物学行为方面具有明确作用。
