• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过核糖体DNA测序鉴定福尔马林固定、石蜡包埋口腔黏膜中的念珠菌种类。

Identification of Candida species in formalin fixed, paraffin wax embedded oral mucosa by sequencing of ribosomal DNA.

作者信息

Williams D W, Wilson M J, Lewis M A, Potts A J

机构信息

Department of Oral Surgery, Medicine and Pathology, Dental School, University of Wales, College of Medicine, Cardiff CF4 4XY.

出版信息

Clin Mol Pathol. 1996 Feb;49(1):M23-8. doi: 10.1136/mp.49.1.m23.

DOI:10.1136/mp.49.1.m23
PMID:16696040
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC408013/
Abstract

Aim-To identify Candida species in formalin fixed, paraffin wax embedded tissue by sequencing candidal rDNA.Methods-Target rDNA sequences were amplified by polymerase chain reaction (PCR) from fresh isolates of Candida and from 18 preserved oral mucosal tissue samples (16 cases of chronic hyperplastic candidiasis and two fibroepithelial polyps), shown histologically to contain Candida. Identification of Candida species within tissue was based on a comparison of the rDNA sequences obtained with those from the fresh isolates of Candida and those present in the GenBank database.Results-The PCR products obtained from 12 of the 18 tissue specimens studied were characteristic of Candida albicans. In two of these cases a second, larger PCR product was obtained and these sequences were characteristic of Candida glabrata.Conclusions-Candidal DNA was amplified successfully from formalin fixed, paraffin wax embedded tissue. Sequencing of the PCR product enabled identification of the Candida species present.

摘要

目的——通过对念珠菌核糖体DNA进行测序，鉴定福尔马林固定、石蜡包埋组织中的念珠菌种类。方法——通过聚合酶链反应（PCR）从念珠菌新鲜分离株以及18份保存的口腔黏膜组织样本（16例慢性增生性念珠菌病和2例纤维上皮息肉）中扩增目标核糖体DNA序列，组织学检查显示这些样本含有念珠菌。基于所获得的核糖体DNA序列与念珠菌新鲜分离株及GenBank数据库中序列的比较，鉴定组织内的念珠菌种类。结果——在所研究的18个组织标本中，12个标本的PCR产物具有白色念珠菌的特征。其中2例还获得了另一种更大的PCR产物，这些序列具有光滑念珠菌的特征。结论——成功从福尔马林固定、石蜡包埋组织中扩增出念珠菌DNA。对PCR产物进行测序能够鉴定出存在的念珠菌种类。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6745/408013/d82f5f4b9075/clinmolpath00012-0029-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6745/408013/49e1f8fb6164/clinmolpath00012-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6745/408013/d82f5f4b9075/clinmolpath00012-0029-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6745/408013/49e1f8fb6164/clinmolpath00012-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6745/408013/d82f5f4b9075/clinmolpath00012-0029-b.jpg

相似文献

1
Identification of Candida species in formalin fixed, paraffin wax embedded oral mucosa by sequencing of ribosomal DNA.通过核糖体DNA测序鉴定福尔马林固定、石蜡包埋口腔黏膜中的念珠菌种类。
Clin Mol Pathol. 1996 Feb;49(1):M23-8. doi: 10.1136/mp.49.1.m23.
2
Immunocytochemical detection of Candida albicans in formalin fixed, paraffin embedded material.在福尔马林固定、石蜡包埋材料中白色念珠菌的免疫细胞化学检测。
J Clin Pathol. 1998 Nov;51(11):857-9. doi: 10.1136/jcp.51.11.857.
3
Sequence analysis of polymerase chain reaction amplified t(14;18) chromosomal breakpoints in formalin fixed, paraffin wax embedded follicular lymphoma.福尔马林固定、石蜡包埋的滤泡性淋巴瘤中聚合酶链反应扩增的t(14;18)染色体断点的序列分析
J Clin Pathol. 1992 Mar;45(3):210-2. doi: 10.1136/jcp.45.3.210.
4
Identification of medically important species by polymerase chain reaction-restriction fragment length polymorphism analysis of the rDNA ITS1 and ITS2 regions.通过核糖体DNA ITS1和ITS2区域的聚合酶链反应-限制性片段长度多态性分析鉴定医学重要物种。
Indian J Med Microbiol. 2017 Oct-Dec;35(4):535-542. doi: 10.4103/ijmm.IJMM_17_102.
5
Rapid and unequivocal differentiation of Candida dubliniensis from other Candida species using species-specific DNA probes: comparison with phenotypic identification methods.使用种特异性DNA探针快速明确地鉴别都柏林念珠菌与其他念珠菌属物种：与表型鉴定方法的比较
Oral Microbiol Immunol. 2003 Dec;18(6):379-88. doi: 10.1046/j.0902-0055.2003.00103.x.
6
Detection of immunoglobulin gene rearrangement of B cell non-Hodgkin's lymphomas and leukemias in fresh, unfixed and formalin-fixed, paraffin-embedded tissue by polymerase chain reaction.通过聚合酶链反应检测新鲜、未固定以及福尔马林固定、石蜡包埋组织中B细胞非霍奇金淋巴瘤和白血病的免疫球蛋白基因重排
Lab Invest. 1993 Jun;68(6):746-57.
7
Detection of mucormycetes and other pathogenic fungi in formalin fixed paraffin embedded and fresh tissues using the extended region of 28S rDNA.使用28S rDNA的扩展区域检测福尔马林固定石蜡包埋组织和新鲜组织中的毛霉菌及其他致病真菌。
Med Mycol. 2017 Jun 1;55(4):385-395. doi: 10.1093/mmy/myw083.
8
Utility of DNA sequencing for direct identification of invasive fungi from fresh and formalin-fixed specimens.DNA 测序在直接鉴定新鲜和福尔马林固定标本中的侵袭性真菌中的应用。
Am J Clin Pathol. 2013 Aug;140(2):203-8. doi: 10.1309/AJCPNSU2SDZD9WPW.
9
Analysis of T cell receptor beta chain CDR3 size using RNA extracted from formalin fixed paraffin wax embedded tissue.使用从福尔马林固定石蜡包埋组织中提取的RNA分析T细胞受体β链互补决定区3（CDR3）的大小
J Clin Pathol. 1997 Oct;50(10):811-4. doi: 10.1136/jcp.50.10.811.
10
Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.用于念珠菌血症诊断的半巢式聚合酶链反应：与培养、抗原检测及用于菌种鉴定的生化方法的比较
J Clin Microbiol. 2002 Jul;40(7):2483-9. doi: 10.1128/JCM.40.7.2483-2489.2002.

引用本文的文献

1
Diagnosis and management of oral candidosis.口腔念珠菌病的诊断与管理
Br Dent J. 2017 Nov 10;223(9):675-681. doi: 10.1038/sj.bdj.2017.886.
2
Detection of Candida in concentrated oral rinse cultures by real-time PCR.通过实时聚合酶链反应检测浓缩口腔冲洗液培养物中的念珠菌。
J Clin Microbiol. 2004 May;42(5):2101-7. doi: 10.1128/JCM.42.5.2101-2107.2004.
3
PCR fingerprinting of Candida albicans associated with chronic hyperplastic candidosis and other oral conditions.与慢性增生性念珠菌病及其他口腔疾病相关的白色念珠菌的聚合酶链反应指纹图谱分析

本文引用的文献

1
Identification of Toxoplasma gondii in formalin-fixed, paraffin-embedded tissue by polymerase chain reaction.通过聚合酶链反应在福尔马林固定、石蜡包埋组织中鉴定刚地弓形虫。
Mod Pathol. 1993 Mar;6(2):185-8.
2
Rapid, polymerase chain reaction-based identification assays for Candida species.基于聚合酶链反应的念珠菌属快速鉴定检测方法。
J Clin Microbiol. 1993 Apr;31(4):904-10. doi: 10.1128/jcm.31.4.904-910.1993.
3
Detection and species identification of mycobacteria in paraffin sections of lung biopsy specimens by the polymerase chain reaction.
J Clin Microbiol. 2001 Nov;39(11):4066-75. doi: 10.1128/JCM.39.11.4066-4075.2001.
4
Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts.多态性内部转录间隔区1 DNA序列可鉴定具有医学重要性的酵母。
J Clin Microbiol. 2001 Nov;39(11):4042-51. doi: 10.1128/JCM.39.11.4042-4051.2001.
通过聚合酶链反应对肺活检标本石蜡切片中的分枝杆菌进行检测和菌种鉴定。
Am J Clin Pathol. 1993 Dec;100(6):643-7. doi: 10.1093/ajcp/100.6.643.
4
A search for mycobacterial DNA in sarcoidosis using the polymerase chain reaction.运用聚合酶链反应在结节病中探寻分枝杆菌DNA。
Am J Clin Pathol. 1994 Jun;101(6):733-7. doi: 10.1093/ajcp/101.6.733.
5
Detection of Candida albicans and other yeasts in blood by PCR.通过聚合酶链反应检测血液中的白色念珠菌和其他酵母菌。
J Clin Microbiol. 1994 Jan;32(1):228-31. doi: 10.1128/jcm.32.1.228-231.1994.
6
Identification of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA.通过聚合酶链反应（PCR）和核糖体DNA基因间隔区的限制性片段长度多态性分析鉴定念珠菌属菌种。
J Clin Microbiol. 1995 Sep;33(9):2476-9. doi: 10.1128/jcm.33.9.2476-2479.1995.
7
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
8
Oral topical steroids and secondary oral candidiasis.
J Oral Med. 1986 Oct-Dec;41(4):223-7, 273.
9
Studies on immunoglobulin gene rearrangement in formalin-fixed, paraffin-embedded pathology specimens.
Am J Pathol. 1988 Mar;130(3):588-94.
10
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。
Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.