Pikkarainen Anna-Liisa
Finnish Institute of Marine Research, Erik Palménin aukio 1, P.O. Box 2, FI-00561 Helsinki, Finland.
Chemosphere. 2006 Dec;65(10):1888-97. doi: 10.1016/j.chemosphere.2006.03.066. Epub 2006 May 15.
Exposure of Baltic Sea perch (Perca fluviatilis) to organic contaminants was investigated by means of liver enzyme activity, measured as ethoxyresorufin-O-deethylase (EROD) activity, and PAH metabolites excreted to bile. Female perch used in the study were caught in coastal waters of the western Gulf of Finland. Reaction conditions were optimised for determination of EROD activity in liver. A high-performance liquid chromatography (HPLC) method with fluorescence detection was developed to separate the interfering fluorescent signal from the signal due to EROD activity. EROD activity in perch varied within a concentration range of 0.30-14 pmol min(-1)mg(-1) protein. Recent PAH exposure was indicated in enhanced levels of the bile metabolite 1-hydroxypyrene, which varied between 213 and 1149 microg kg(-1). No correlation was indicated between hepatic EROD activity and concentration of 1-hydroxypyrene.
通过测定肝脏酶活性（以乙氧基异吩恶唑酮 - O - 脱乙基酶（EROD）活性衡量）以及排泄到胆汁中的多环芳烃（PAH）代谢物，研究了波罗的海鲈鱼（河鲈）对有机污染物的暴露情况。该研究中使用的雌鱼捕获于芬兰湾西部沿海水域。对测定肝脏中EROD活性的反应条件进行了优化。开发了一种带有荧光检测的高效液相色谱（HPLC）方法，以将干扰荧光信号与EROD活性产生的信号分离。鲈鱼的EROD活性在0.30 - 14 pmol min(-1)mg(-1)蛋白质的浓度范围内变化。胆汁代谢物1 - 羟基芘水平升高表明近期接触了PAH，其含量在213至1149 microg kg(-1)之间变化。肝脏EROD活性与1 - 羟基芘浓度之间未显示出相关性。
