Department of Marine Ecology, National Environmental Research Institute, Aarhus University, Frederiksborgvej 399, DK-4000 Roskilde, Denmark.
Mar Environ Res. 2012 Apr;75:45-53. doi: 10.1016/j.marenvres.2011.09.005. Epub 2011 Sep 25.
Eelpouts (Zoarces viviparus) sampled at surveillance stations during the fall of 2007 and spring 2008 in different Danish coastal areas, were studied for biomarkers of polycyclic aromatic hydrocarbons (PAHs) exposure and effects. Two analytical techniques, synchronous fluorescence spectrometry (SFS) and high-performance liquid chromatography with fluorescence detection (HPLC/F), were applied for detecting PAH metabolites in bile and urine. CYP1A activity, in this study regarded as potential biomarker of effect, was measured as 7-ethoxyresorufin-O-deethylase (EROD) activity in liver of eelpouts from different stations. Biliary PAH metabolite measurements were used for monitoring the environmental PAH load at the surveillance stations. There was found significant difference in biliary PAH metabolite content between sexes with male fish containing higher concentrations of PAH metabolites than females. The urinary PAH metabolite content did not show the same spatial trends as biliary PAH metabolites. However, fish from Aarhus Bight and Vejle Fjord had significantly higher levels of PAH metabolites in both urine and bile compared to the reference station Agersø. Normalisation methods applied for bile and urine matrices did not have any effect or only slightly reduced the coefficients of variation in data sets. The CYP1A activity in eelpout liver did not show the same spatial distribution trends between sampling sites as did biliary or urinary PAH metabolite contents. Male eelpouts showed significantly higher CYP1A activity than females in fall sampling period but there were no differences found in the spring period. General comparison between both seasons showed that eelpouts sampled in the fall had significantly higher CYP1A activity than fish sampled during spring season. Overall, the results of this study describe selected biomarker responses in eelpouts to environmental PAH load at the different areas along Danish coasts.
2007 年秋季和 2008 年春季，在丹麦不同沿海地区的监测站采集了拟庸鲽（Zoarces viviparus）样本，以研究其多环芳烃（PAHs）暴露和效应的生物标志物。本研究采用同步荧光光谱法（SFS）和高效液相色谱荧光检测法（HPLC/F）两种分析技术，检测胆汁和尿液中的 PAH 代谢物。本研究中，CYP1A 活性被视为潜在的效应生物标志物，通过不同监测站拟庸鲽肝脏中的 7-乙氧基resorufin-O-脱乙基酶（EROD）活性进行测量。胆汁 PAH 代谢物的测量用于监测监测站的环境 PAH 负荷。研究发现，雌雄鱼之间的胆汁 PAH 代谢物含量存在显著差异，雄性鱼中 PAH 代谢物的浓度高于雌性鱼。尿液 PAH 代谢物含量没有表现出与胆汁 PAH 代谢物相同的空间趋势。然而，与参考站 Agersø相比，奥胡斯湾和维勒湾的鱼在尿液和胆汁中均具有更高水平的 PAH 代谢物。应用于胆汁和尿液基质的归一化方法对数据集的变异系数没有任何影响或仅略有降低。拟庸鲽肝脏中的 CYP1A 活性与胆汁或尿液 PAH 代谢物含量之间的采样点空间分布趋势并不相同。在秋季采样期间，雄性拟庸鲽的 CYP1A 活性明显高于雌性，但在春季期间未发现差异。两个季节的综合比较表明，秋季采样的拟庸鲽的 CYP1A 活性明显高于春季采样的鱼。总体而言，本研究结果描述了丹麦沿海不同地区环境 PAH 负荷对拟庸鲽的选定生物标志物反应。
