Tripathi B N, Periasamy Sivakumar, Paliwal O P, Singh Nem
Division of Pathology, Indian Veterinary Research Institute, Izatnagar 243122, UP, India.
Vet Microbiol. 2006 Aug 25;116(1-3):129-37. doi: 10.1016/j.vetmic.2006.03.017. Epub 2006 May 15.
Comparative efficacy of an IS900 tissue PCR, bacterial culture, johnin, agar-gel immunodiffusion (AGID) and absorbed-ELISA tests was investigated in 43 goats naturally infected with paratuberculosis. On histological examination, tissue sections from all animals showed typical granulomatous inflammatory changes. The lesions were classified as multibacillary (MB) (n=30), which had diffuse granulomatous lesions with abundant acid-fast bacilli (AFB), and paucibacillary (PB) (n=13), which had focal or multifocal granulomatous lesions with few AFB. The sensitivities of johnin test, tissue culture, faecal culture, tissue PCR, AGID and ELISA were 68% (17/25), 100% (30/30), 84.6% (22/26), 100% (30/30), 96.2% (25/26) and 100% (26/26) in MB goats, and 88.8 (8/9), 46.1% (8/13), 40% (4/10), 61.5% (8/13), 50% (5/10), and 70% (7/10) in PB goats, respectively. Except for the johnin test, which showed higher sensitivity in PB goats, all other tests displayed significantly higher sensitivities in MB goats. The results indicate the usefulness of tissue PCR, culture and serological tests in the diagnosis of clinically affected paratuberculous goats, especially with multibacillary pathology.
在43只自然感染副结核病的山羊中，研究了IS900组织聚合酶链反应（PCR）、细菌培养、副结核菌素、琼脂凝胶免疫扩散（AGID）和吸附酶联免疫吸附测定（ELISA）试验的比较效能。组织学检查显示，所有动物的组织切片均呈现典型的肉芽肿性炎症变化。病变分为多菌型（MB）（n = 30），其具有弥漫性肉芽肿病变且抗酸杆菌（AFB）丰富，以及少菌型（PB）（n = 13），其具有局灶性或多灶性肉芽肿病变且AFB较少。在MB型山羊中，副结核菌素试验、组织培养、粪便培养、组织PCR、AGID和ELISA的敏感性分别为68%（17/25）、100%（30/30）、84.6%（22/26）、100%（30/30）、96.2%（25/26）和100%（26/26）；在PB型山羊中，敏感性分别为88.8%（8/9）、46.1%（8/13）、40%（4/10）、61.5%（8/13）、50%（5/10）和70%（7/10）。除副结核菌素试验在PB型山羊中显示出较高敏感性外，所有其他试验在MB型山羊中的敏感性均显著更高。结果表明，组织PCR、培养和血清学检测在诊断临床受影响的副结核山羊中有用，尤其是对于有多菌型病理的山羊。
