Ceelie H, Dinkelaar R B, van Gelder W
Department of Clinical Chemistry, Albert Schweitzer Ziekenhuis, Dordrecht, The Netherlands.
J Clin Pathol. 2007 Jan;60(1):72-9. doi: 10.1136/jcp.2005.035402. Epub 2006 May 12.
Differential counting of peripheral blood cells is an important diagnostic tool. Yet, this technique requires highly trained staff, is labour intensive and has limited statistical reliability. A recent development in this field was the introduction of automated peripheral blood differential counting systems. These computerised systems provide an automated morphological analysis of peripheral blood films, including a preclassification of both red and white cells (RBCs and WBCs, respectively).
To investigate the ability of two automated microscopy systems to examine peripheral blood smears.
Two automated microscopy systems, the Cellavision Diffmaster Octavia (Octavia) and Cellavision DM96 (DM96), were evaluated.
The overall preclassification accuracy values for the Octavia and the DM96 systems were 87% and 92%, respectively. Evaluation of accuracy (WBC analysis) showed good correlation for both automated systems when compared with manual differentiation. Total analysis time (including post classification) was 5.4 min/slide for the Octavia and 3.2 min/slide for the DM96 (100 WBC/slide) system. The DM96 required even less time than manual differentiation by an experienced biomedical scientist.
The Octavia and the DM96 are automated cell analysis systems capable of morphological classification of RBCs and WBCs in peripheral blood smears. Classification accuracy depends on the type of pathological changes in the blood sample. Both systems operate most effectively in the analysis of non-pathological blood samples.
外周血细胞分类计数是一项重要的诊断工具。然而，这项技术需要训练有素的工作人员，劳动强度大且统计可靠性有限。该领域最近的一项进展是引入了自动外周血分类计数系统。这些计算机化系统可对外周血涂片进行自动形态学分析，包括对红细胞和白细胞（分别为RBC和WBC）进行预分类。
研究两种自动显微镜系统检查外周血涂片的能力。
对两种自动显微镜系统，即Cellavision Diffmaster Octavia（Octavia）和Cellavision DM96（DM96）进行评估。
Octavia和DM96系统的总体预分类准确率分别为87%和92%。准确性评估（白细胞分析）显示，与手工分类相比，两种自动系统的相关性良好。Octavia系统的总分析时间（包括分类后）为每张涂片5.4分钟，DM96（每涂片100个白细胞）系统为每张涂片3.2分钟。DM96所需时间甚至比经验丰富的生物医学科学家进行手工分类所需时间还要少。
Octavia和DM96是能够对外周血涂片红细胞和白细胞进行形态学分类的自动细胞分析系统。分类准确性取决于血样中病理变化的类型。两种系统在分析非病理血样时效果最佳。
