Andreote Fernando D, Lacava Paulo T, Gai Cláudia S, Araújo Welington L, Maccheroni Walter, van Overbeek Leonard S, van Elsas Jan Dirk, Azevedo João Lúcio
Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, ESALQ/USP, Piracicaba, Brazil.
Can J Microbiol. 2006 May;52(5):419-26. doi: 10.1139/w05-142.
Over the last few years, endophytic bacterial communities associated with citrus have been studied as key components interacting with Xylella fastidiosa. In this study, we investigated the possible interaction between the citrus endophyte Methylobacterium mesophilicum SR1.6/6 and X. fastidiosa in model plants such as Catharanthus roseus (Madagaskar periwinkle) and Nicotiana clevelandii (Clevelands tobacco). The aim of this study was to establish the fate of M. mesophilicum SR1.6/6 after inoculation of C. roseus and N. clevelandii plants, using PCR-DGGE (polymerase chain reaction--denaturing gradient gel electrophoresis) and plating techniques. Shifts in the indigenous endophytic bacterial communities were observed in plants inoculated with strain SR1.6/6, using specific primers targeting alpha- and beta-Proteobacteria. Cells of strain SR1.6/6 were observed in a biofilm structure on the root and hypocotyl surfaces of in vitro seedlings inoculated with M. mesophilicum SR1.6/6. This emphasizes the importance of these tissues as main points of entrance for this organism. The results showed that C. roseus and N. clevelandii could be used as model plants to study the interaction between M. mesophilicum and X. fastidiosa.
在过去几年中，与柑橘相关的内生细菌群落作为与木质部难养菌相互作用的关键组成部分受到了研究。在本研究中，我们在长春花（马达加斯加长春花）和克利夫兰烟草等模式植物中研究了柑橘内生菌嗜中温甲基杆菌SR1.6/6与木质部难养菌之间可能的相互作用。本研究的目的是通过聚合酶链反应 - 变性梯度凝胶电泳（PCR-DGGE）和平板接种技术，确定在接种长春花和克利夫兰烟草植物后嗜中温甲基杆菌SR1.6/6的命运。使用靶向α-和β-变形菌纲的特异性引物，在接种菌株SR1.6/6的植物中观察到了本地内生细菌群落的变化。在用嗜中温甲基杆菌SR1.6/6接种的离体幼苗的根和下胚轴表面的生物膜结构中观察到了菌株SR1.6/6的细胞。这强调了这些组织作为该生物体主要进入点的重要性。结果表明，长春花和克利夫兰烟草可作为模式植物来研究嗜中温甲基杆菌与木质部难养菌之间的相互作用。
