Badji Boubekeur, Mostefaoui Abdellah, Sabaou Nasserdine, Lebrihi Ahmed, Mathieu Florence, Seguin Elisabeth, Tillequin François
Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la Biomasse, Ecole Normale Supérieure de Kouba, B.P. 92, 16 050, Vieux-Kouba, Alger, Algérie.
J Ind Microbiol Biotechnol. 2007 Jun;34(6):403-12. doi: 10.1007/s10295-007-0210-z. Epub 2007 Feb 22.
An actinomycete strain NM94 was isolated from a Saharan soil sample by a dilution agar plating method using chitin-vitamins B medium supplemented with penicillin. The strain presented the morphological and chemical characteristics of the genus Nonomuraea. On the basis of 16S rDNA analysis and physiological tests, this isolate was found to be quite different from the known species of Nonomuraea and might be new. The strain NM94 secreted several antibiotics on yeast extract malt extract glucose medium that were active against some Gram-positive bacteria, yeast, and fungi. The antibiotics were extracted with dichloromethane and detected by bioautography on silica gel plates using Mucor ramannianus and Bacillus subtilis as the test organisms. Among these antibiotics, a complex called 94A showed interesting antifungal activity. It was selected and purified by reverse-phase HPLC. This complex was composed of five compounds. Spectroscopic studies by infrared, mass, and (1)H NMR of the compounds were carried out. Initial results showed that these molecules differed from the known antibiotics produced by other Nonomuraea species.
通过使用添加了青霉素的几丁质 - 维生素B培养基的稀释琼脂平板法，从撒哈拉沙漠土壤样本中分离出一株放线菌菌株NM94。该菌株呈现出野野村菌属的形态和化学特征。基于16S rDNA分析和生理测试，发现该分离株与野野村菌属的已知物种有很大不同，可能是新物种。菌株NM94在酵母提取物麦芽提取物葡萄糖培养基上分泌了几种抗生素，这些抗生素对一些革兰氏阳性细菌、酵母和真菌具有活性。用二氯甲烷提取抗生素，并使用拉曼毛霉和枯草芽孢杆菌作为测试生物在硅胶板上通过生物自显影法进行检测。在这些抗生素中，一种名为94A的复合物表现出有趣的抗真菌活性。通过反相高效液相色谱法对其进行了分离和纯化。该复合物由五种化合物组成。对这些化合物进行了红外、质谱和¹H NMR光谱研究。初步结果表明，这些分子与其他野野村菌属物种产生的已知抗生素不同。
