Sarkonen Nanna, Könönen Eija, Eerola Erkki, Könönen Mauno, Jousimies-Somer Hannele, Laine Pekka
Anaerobe Reference Laboratory, Department of Microbiology, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
Anaerobe. 2005 Aug;11(4):231-7. doi: 10.1016/j.anaerobe.2005.01.002. Epub 2005 Apr 7.
In the oral cavity, Actinomyces form a fundamental component of the indigenous microflora, being among initial colonizers in polymicrobial biofilms. However, some differences may exist between different species in terms of their attachment not only to teeth but also to biomaterials. In this study we investigated the distribution of Actinomyces in 33 dental implant fixtures explanted from 17 patients. The identification was based on comprehensive biochemical testing and gas-liquid chromatography and when needed, 16S rRNA sequencing. Actinomyces was the most prevalent bacterial genus in these failed implants, colonizing 31/33 (94%) of the fixtures. Proportions of Actinomyces growth of the total bacterial growth in the Actinomyces-positive fixtures varied from 0.01% up to 75%. A. odontolyticus was the most common Actinomyces finding, present in 26/31 (84%) Actinomyces-positive fixtures. Actinomyces naeslundii and A. viscosus were both detected in 10/31 (32%) and A. israelii in 7/31 (23%) fixtures. Other Actinomyces species, including A. georgiae, A. gerencseriae and A. graevenitzii, were detected less frequently. Our results suggest that Actinomyces species are frequent colonizers on failed implant surfaces, where A. odontolyticus was the far most prominent Actinomyces species.
在口腔中，放线菌是固有微生物群落的基本组成部分，是多微生物生物膜的初始定植菌之一。然而，不同种类的放线菌在附着于牙齿以及生物材料方面可能存在一些差异。在本研究中，我们调查了从17名患者体内取出的33个牙种植体固定装置中放线菌的分布情况。鉴定基于全面的生化测试和气液色谱法，必要时还采用16S rRNA测序。放线菌是这些失败种植体中最常见的细菌属，在33个固定装置中的31个（94%）上定植。在放线菌阳性的固定装置中，放线菌生长占总细菌生长的比例从0.01%到75%不等。溶牙放线菌是最常见的放线菌，在31个放线菌阳性固定装置中的26个（84%）中被发现。内氏放线菌和黏性放线菌在31个固定装置中的10个（32%）中均被检测到，以色列放线菌在31个固定装置中的7个（23%）中被检测到。其他放线菌种类，包括佐治亚放线菌、格氏放线菌和格氏放线菌，检测频率较低。我们的结果表明，放线菌是失败种植体表面的常见定植菌，其中溶牙放线菌是最主要的放线菌种类。
