Laboratoire des Multimatériaux et Interfaces, Université Lyon 1, Université de Lyon, Villeurbanne, France.
PLoS One. 2012;7(7):e39367. doi: 10.1371/journal.pone.0039367. Epub 2012 Jul 31.
Complications in dentistry and orthopaedic surgery are mainly induced by peri-implant bacterial infections and current implant devices do not prevent such infections. The coating of antibacterial molecules such as chitosan on its surface would give the implant bioactive properties. The major challenge of this type of coating is the attachment of chitosan to a metal substrate. In this study, we propose to investigate the functionalization of titanium with chitosan via a silanation. Firstly, the surface chemistry and mechanical properties of such coating were evaluated. We also verified if the coated chitosan retained its biocompatibility with the peri-implant cells, as well as its antibacterial properties. FTIR and Tof-SIMS analyses confirmed the presence of chitosan on the titanium surface. This coating showed great scratch resistance and was strongly adhesive to the substrate. These mechanical properties were consistent with an implantology application. The Chitosan-coated surfaces showed strong inhibition of Actinomyces naeslundii growth; they nonetheless showed a non significant inhibition against Porphyromonas gingivalis after 32 hours in liquid media. The chitosan-coating also demonstrated good biocompatibility to NIH3T3 fibroblasts. Thus this method of covalent coating provides a biocompatible material with improved bioactive properties. These results proved that covalent coating of chitosan has significant potential in biomedical device implantation.
口腔医学和矫形外科的并发症主要是由种植体周围细菌感染引起的，而目前的种植体设备并不能预防这种感染。在其表面涂覆抗菌分子如壳聚糖将赋予植入物生物活性。这种涂层的主要挑战是将壳聚糖附着到金属基底上。在本研究中，我们建议通过硅烷化来研究壳聚糖对钛的功能化。首先，评估了这种涂层的表面化学和机械性能。我们还验证了涂覆的壳聚糖是否保留了其与种植体周围细胞的生物相容性以及其抗菌性能。FTIR 和 ToF-SIMS 分析证实了壳聚糖存在于钛表面。这种涂层具有很强的耐划伤性，并且与基底具有很强的粘附性。这些机械性能与植入物应用一致。壳聚糖涂层表面强烈抑制奈瑟放线菌的生长；然而，在液体培养基中 32 小时后，对牙龈卟啉单胞菌的抑制作用不显著。壳聚糖涂层对 NIH3T3 成纤维细胞也表现出良好的生物相容性。因此，这种共价涂层方法提供了一种具有改善的生物活性的生物相容性材料。这些结果证明壳聚糖的共价涂层在生物医学设备植入中有很大的潜力。
