Department of Gerodontology and Oral Pathology, University of Medical Sciences Poznan, Poznan, Poland.
Physiol Res. 2021 Jul 12;70(3):413-423. doi: 10.33549/physiolres.934582. Epub 2021 May 12.
The objective of this study was to evaluate and compare titanium surfaces: machined (MA); sintered ceramic-blasted (HAS); sintered ceramic-blasted and acid-etched (HAS DE) and to determine the effects of surface topography, roughness and chemical composition on human osteoblast cell reaction. Titanium surface samples were analyzed with respect to surface chemical composition, topography, and roughness. The effects of material surface characteristics on osteoblasts was examined by analyzing osteoblast morphology, viability and differentiation. Osteoblasts cultured on these materials had attached, spread and proliferated on every sample. The viability of osteoblasts cultured on HAS and HAS DE samples increased more intensively in time comparing to MA sample. The viability of osteoblast cultured on HAS samples increased more intensively in the early phases of culture while for cells cultured on HAS DE the cells viability increased later in time. Alkaline phosphate activity was the highest for the cells cultured on HAS sample and statistically higher than for the MA sample. The least activity occurred on the smooth MA sample along with the rougher HAS DE samples. All the examined samples were found to be biocompatible, as indicated by cell attachment, proliferation, and differentiation. Titanium surfaces modification improved the dynamics of osteoblast viability increase. Osteoblast differentiation was found to be affected by the etching procedure and presence of Ca and P on the surface.
机械加工（MA）；烧结陶瓷喷砂（HAS）；烧结陶瓷喷砂和酸蚀（HAS DE），并确定表面形貌、粗糙度和化学成分对人成骨细胞反应的影响。对钛表面样品进行了表面化学成分、形貌和粗糙度分析。通过分析成骨细胞的形态、活力和分化，研究了材料表面特性对成骨细胞的影响。成骨细胞在这些材料上培养后，能够附着、铺展和增殖。与 MA 样品相比，在 HAS 和 HAS DE 样品上培养的成骨细胞的活力随时间增加得更加剧烈。在培养的早期阶段，在 HAS 样品上培养的成骨细胞的活力增加得更加剧烈，而在 HAS DE 样品上培养的成骨细胞的活力增加得更晚。在 HAS 样品上培养的细胞的碱性磷酸酶活性最高，且与 MA 样品相比具有统计学意义。在光滑的 MA 样品上，以及在更粗糙的 HAS DE 样品上，碱性磷酸酶活性最低。所有被检测的样品都被认为是生物相容的，这表明细胞附着、增殖和分化。钛表面改性提高了成骨细胞活力增加的动力学。发现成骨细胞分化受蚀刻工艺和表面 Ca 和 P 的影响。
