Mabilleau G, Bourdon S, Joly-Guillou M L, Filmon R, Baslé M F, Chappard D
INSERM, EMI 0335-LHEA, Faculté de Médecine, F-49045 Angers Cedex, France.
Acta Biomater. 2006 Jan;2(1):121-9. doi: 10.1016/j.actbio.2005.09.004. Epub 2005 Oct 25.
Titanium is widely used in dental implantology and orthopaedics due to its excellent corrosion resistance and mechanical properties. However, it has been reported that Ti is sensitive to F(-), H(2)O(2) and lactic acid. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) were used to investigate the corrosion resistance of CP-Ti disks after 9 days immersion in different test solutions, based on artificial saliva containing F(-) (0.5% and 2.5%), H(2)O(2) (0.1% and 10%) and/or lactic acid. Because activated macrophages and bacteria can also release locally some of these oxidative compounds, we investigated the role of these cells when plated onto titanium disks. The surface roughness (R(a)) was highly increased when titanium disks were immersed in artificial saliva containing F(-), H(2)O(2) and lactic acid. After 21 days of cell culture, R(a) was significantly increased on disks incubated with activated-J774.2 cells or Streptococcus mitis. AFM appeared to be more sensitive than SEM in evaluating the corrosion of the titanium. Chemical species, either environmental or those released by macrophages and bacteria, can provoke a marked attack of the titanium surface.
钛因其优异的耐腐蚀性和机械性能而广泛应用于牙种植学和矫形外科学领域。然而，据报道钛对氟离子（F(-)）、过氧化氢（H₂O₂）和乳酸敏感。基于含氟离子（0.5%和2.5%）、过氧化氢（0.1%和10%）和/或乳酸的人工唾液，使用原子力显微镜（AFM）和扫描电子显微镜（SEM）研究了商业纯钛（CP-Ti）盘在不同测试溶液中浸泡9天后的耐腐蚀性。由于活化的巨噬细胞和细菌也可局部释放其中一些氧化化合物，我们研究了将这些细胞接种到钛盘上时的作用。当钛盘浸泡在含氟离子、过氧化氢和乳酸的人工唾液中时，表面粗糙度（R(a)）显著增加。细胞培养21天后，与活化的J774.2细胞或缓症链球菌共同孵育的钛盘上的R(a)显著增加。在评估钛的腐蚀方面，AFM似乎比SEM更敏感。环境中的化学物质或巨噬细胞和细菌释放的化学物质均可引发对钛表面的显著侵蚀。
