Sadeyen Jean-Rémy, Trotereau Jérôme, Protais Jocelyne, Beaumont Catherine, Sellier Nadine, Salvat Gilles, Velge Philippe, Lalmanach Anne-Christine
INRA, Unité Infectiologie Animale et Santé Publique, IASP213, Centre de Recherche de Tours, 37380 Nouzilly, France.
Microbes Infect. 2006 Apr;8(5):1308-14. doi: 10.1016/j.micinf.2005.12.014. Epub 2006 Mar 15.
Salmonellosis is one of the main causes of food-borne poisoning due to the consumption of contaminated poultry products. In the flocks, Salmonella is able to persist in the digestive tract of birds for weeks without triggering any symptom. In order to identify molecules and genes involved in the mechanism of host resistance to intestinal carrier-state, two different inbred lines of laying hens were orally inoculated with Salmonella Enteritidis. Bacterial colonization and host gene expression were measured in the caecum and its sentinel lymphoid tissue, respectively. Significantly increased expression of chemokine, anti-infectious cytokine, bacterial receptor, antimicrobial mediator and particularly, defensin genes was observed in the line carrying a lower level of bacteria in the caecum. These innate immunity molecules were either constitutively or inductively highly expressed in resistant adult birds and thus present candidate genes to play an important role in the host defence against Salmonella colonization.
沙门氏菌病是因食用受污染的家禽产品而导致食源性中毒的主要原因之一。在鸡群中，沙门氏菌能够在禽类消化道中持续存在数周而不引发任何症状。为了鉴定参与宿主对肠道携带状态抗性机制的分子和基因，用肠炎沙门氏菌对两个不同的近交系蛋鸡进行口服接种。分别在盲肠及其前哨淋巴组织中测量细菌定植和宿主基因表达。在盲肠中细菌水平较低的品系中，观察到趋化因子、抗感染细胞因子、细菌受体、抗菌介质，特别是防御素基因的表达显著增加。这些先天免疫分子在抗性成年禽类中要么组成性地要么诱导性地高表达，因此是在宿主抵御沙门氏菌定植中发挥重要作用的候选基因。
