Lee S G, Littau V, Lipmann F
J Cell Biol. 1975 Aug;66(2):233-42. doi: 10.1083/jcb.66.2.233.
The induction and localization of tyrocidine-synthesizing enzymes is shown to be parallel, during growth of Bacillus brevis (ATCC 8185, American Type Culture Collection, Rockville, Md.), with the induction of uptake of constitutive amino acids and of components of pantetheine, a coenzyme of tyrocidine synthesis. Antibiotic synthesis appears at the end of logarithmic growth when the first soluble enzymes may be obtained from homogenates. During this period, binding proteins for metabolite uptake were isolated by intensive sonication which, when studied by chromatography, were identified by the appearance of low molecular weight fractions binding the radioactively marked metabolites; their induction was prevented by addition of rifampicin. The major purpose of this study was a comparison of antibiotic production and sporulation, the progress of which was followed by electron microscopy. The onset of tyrocidine synthesis and metabolite uptake coincided with the appearance of septum formation indicating that sporulation had progressed to stage II. With the progress of spore encapsulation, the tyrocidine production migrated from the soluble fraction into the forespore, terminating with the separation of forespores from the sporangium membrane. The resulting concentration of antibiotic in the forespore may indicate its function in sporulation, the nature of which, however, was not explored.
在短短芽孢杆菌（美国典型培养物保藏中心，马里兰州罗克维尔，ATCC 8185）的生长过程中，短杆菌肽合成酶的诱导和定位与组成型氨基酸的摄取以及泛酰巯基乙胺（短杆菌肽合成的一种辅酶）成分的摄取诱导是平行的。抗生素合成出现在对数生长期结束时，此时可以从匀浆中获得第一批可溶性酶。在此期间，通过强力超声处理分离出用于代谢物摄取的结合蛋白，当通过色谱法研究时，通过出现结合放射性标记代谢物的低分子量级分来鉴定它们；添加利福平可阻止它们的诱导。本研究的主要目的是比较抗生素生产和孢子形成，并通过电子显微镜观察其进展情况。短杆菌肽合成和代谢物摄取的开始与隔膜形成的出现同时发生，表明孢子形成已进展到第二阶段。随着孢子包封的进行，短杆菌肽的产生从可溶性部分迁移到前孢子中，以前孢子与孢子囊膜分离而结束。前孢子中抗生素的最终浓度可能表明其在孢子形成中的作用，但其性质尚未探究。
