Lee S G, Lipmann F
Proc Natl Acad Sci U S A. 1977 Jan;74(1):163-7. doi: 10.1073/pnas.74.1.163.
A glucose binding fraction was obtained by sucrose gradient centrifugation and Sephadex G-200 chromatopgraphy from confluent normal cells and Rous sarcoma virus-transformed fibroblasts. It was more or less loosely bound to the membrane fraction, most strongly in sarcoma cells, and most loosely in slowly growing confluent fibroblasts. In an average of three determinations, the content of binding factor was increased 2.5-fold in transformed cells, and compared reasonably well to a nearly 4-fold increase in glucose uptake. The addition of 5 mug/ml of purified glucose binding factor to the overlaying fluid in the 100-mm plates increased 7-flod the low glucose uptake of starved fibroblasts. The stimulation was an additive increment to the known stimulation by calf serum.
通过蔗糖梯度离心和Sephadex G - 200层析从汇合的正常细胞和劳氏肉瘤病毒转化的成纤维细胞中获得了葡萄糖结合部分。它与膜部分的结合或多或少比较松散，在肉瘤细胞中结合最强，在缓慢生长的汇合成纤维细胞中结合最松散。在平均三次测定中，转化细胞中结合因子的含量增加了2.5倍，与葡萄糖摄取增加近4倍相当吻合。向100毫米培养皿的覆盖液中添加5微克/毫升纯化的葡萄糖结合因子，使饥饿成纤维细胞的低葡萄糖摄取增加了7倍。这种刺激是对小牛血清已知刺激的累加增加。
