正常前列腺组织中人疱疹病毒8型（HHV-8）的检测
Detection of human herpesvirus 8 (HHV-8) in normal prostates.
作者信息
Montgomery J D, Jacobson L P, Dhir R, Jenkins F J
机构信息
Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA.
出版信息
Prostate. 2006 Sep 1;66(12):1302-10. doi: 10.1002/pros.20459.
BACKGROUND
Human herpesvirus 8 (HHV-8) DNA has been detected in semen and prostatic tissues in some, but not all reports. We have analyzed prostate tissues from HHV-8 seropositive men for the expression of viral proteins and determined if expression of these proteins are associated with increased inflammation.
METHODS
Paraffin sections of non-cancerous prostates from HHV-8 seropositive (n = 16) and seronegative (n = 2) men who died with AIDS were screened for expression of three viral proteins by immunohistochemistry. Levels of inflammation were determined by expression of CD68 and CD20. Cellular proliferation was determined by expression of Ki67.
RESULTS
Among the 16 HHV-8 seropositive cases, 68.9% (11/16) (95% C.I. = 0.41-0.89) were positive for HHV-8 protein expression, while the 2 seronegative patients showed no HHV-8 protein expression. There was increased inflammation among HHV-8 positive prostates.
CONCLUSIONS
These results demonstrate that HHV-8 is present in normal prostates of HIV-infected men and the expression of viral proteins is associated with increased localized inflammation.
背景
在一些但并非所有报告中，已在精液和前列腺组织中检测到人类疱疹病毒8（HHV - 8）DNA。我们分析了HHV - 8血清反应阳性男性的前列腺组织中病毒蛋白的表达情况，并确定这些蛋白的表达是否与炎症增加相关。
方法
通过免疫组织化学对死于艾滋病的HHV - 8血清反应阳性（n = 16）和血清反应阴性（n = 2）男性的非癌性前列腺石蜡切片进行三种病毒蛋白表达的筛查。通过CD68和CD20的表达确定炎症水平。通过Ki67的表达确定细胞增殖情况。
结果
在16例HHV - 8血清反应阳性病例中，68.9%（11/16）（95%置信区间 = 0.41 - 0.89）HHV - 8蛋白表达呈阳性，而2例血清反应阴性患者未显示HHV - 8蛋白表达。HHV - 8阳性的前列腺中炎症增加。
结论
这些结果表明，HHV - 8存在于HIV感染男性的正常前列腺中，病毒蛋白的表达与局部炎症增加相关。
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