Xu Xia, Zhao Jinan, Li Zhengjun, Lin Fang, He Zhuangzhi, Zhang Tingyou
The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Apr;23(2):362-5.
Nano-fumed silica derivative with quaternary ammonium group was synthesized and the antimicrobial activity was investigated. The nano-fumed silica derivative was investigated by Fourier-transform infrared spectroscopy (FTIR). The zeta potential and the size of the nano-fumed silica derivative were measured. The antimicrobial properties of the nano-fumed silica derivative against selected microorganisms were tested by the quantitative suspension method. The zeta potential showed that the isoelectric points of nano-fumed silica and modified nano-fumed silica are pH=4. 8 and pH =10.5-10.8, respectively, and the shift of the isoelectric point is due to the quaternary ammonium salt. The obtained nano-fumed silica derivative inhibited the growth of Gram-negative (Escherichia coli), Gram-positive bacteria (Staphylococcus aureus), and fungus (Candida albicans). The inhibiting effect of nano-fumed silica derivative on the microorganisms varied with the time of exposure. The bacteriostatic rates were noted to be 99.99%, 99.99% and 94.12% after 15 minutes' exposure, respectively. Thus the results indicate that nano-fumed silica derivative with quaternary ammonium group has significant inhibitory effect on the growth of bacteria.
合成了带有季铵基团的纳米气相二氧化硅衍生物，并对其抗菌活性进行了研究。通过傅里叶变换红外光谱（FTIR）对纳米气相二氧化硅衍生物进行了研究。测量了纳米气相二氧化硅衍生物的zeta电位和粒径。采用定量悬浮法测试了纳米气相二氧化硅衍生物对选定微生物的抗菌性能。zeta电位表明，纳米气相二氧化硅和改性纳米气相二氧化硅的等电点分别为pH = 4.8和pH = 10.5 - 10.8，等电点的移动是由于季铵盐的作用。所得到的纳米气相二氧化硅衍生物抑制了革兰氏阴性菌（大肠杆菌）、革兰氏阳性菌（金黄色葡萄球菌）和真菌（白色念珠菌）的生长。纳米气相二氧化硅衍生物对微生物的抑制作用随暴露时间而变化。暴露15分钟后，抑菌率分别为99.99%、99.99%和94.12%。因此，结果表明带有季铵基团的纳米气相二氧化硅衍生物对细菌生长具有显著的抑制作用。
