Kim Yoon Seok, Kim Hyung Woo, Lee Sun Hee, Shin Kyoung Sook, Hur Hyoung Woo, Rhee Young Ha
Department of Microbiology, Chungnam National University, Daejeon 305-764, South Korea.
Int J Biol Macromol. 2007 Jun 1;41(1):36-41. doi: 10.1016/j.ijbiomac.2006.12.004. Epub 2006 Dec 27.
Alginate-quaternary ammonium complex beads with antimicrobial activity were prepared by the reaction of sodium alginate (SA) with 3-(trimethoxysilyl)propyl-octadecyldimethylammonium chloride (TSA) in acid solution, followed by crosslinking with CaCl(2). FTIR spectroscopy analysis showed that the resulting complex was formed mainly through covalent bonds between the hydroxyl groups of SA and the methoxysilyl groups of TSA. The complex beads exhibited a maximum swelling of 20% in water at 37 degrees C and were not hydrolyzed in water during experiments lasting for 30 days. The in vitro antimicrobial activity of the complex beads was evaluated against four species of bacteria and fungi. The test microorganisms were completely eliminated within 20 min when treated with 5% (v/v) complex beads, which showed a wide spectrum of excellent antimicrobial activity. The antimicrobial activity of the complex beads was retained after 10 cycles of washing and drying. The present results indicate that these SA-TSA complex beads are a new type of insoluble cationic polymer that can kill or remove microorganisms in water by mere contact without releasing the reactive agent.
通过在酸性溶液中使海藻酸钠（SA）与3-（三甲氧基甲硅烷基）丙基十八烷基二甲基氯化铵（TSA）反应，然后与CaCl₂交联，制备了具有抗菌活性的海藻酸盐-季铵络合物珠。傅里叶变换红外光谱分析表明，所得络合物主要通过SA的羟基与TSA的甲氧基甲硅烷基之间的共价键形成。该络合物珠在37℃水中的最大溶胀率为20%，并且在持续30天的实验过程中在水中未发生水解。针对四种细菌和真菌评估了该络合物珠的体外抗菌活性。用5%（v/v）的络合物珠处理时，测试微生物在20分钟内被完全清除，这表明其具有广泛的优异抗菌活性。经过10次洗涤和干燥循环后，络合物珠仍保留抗菌活性。目前的结果表明，这些SA-TSA络合物珠是一种新型的不溶性阳离子聚合物，其可以通过简单接触杀死或去除水中的微生物而不释放反应剂。
