Xu Xia, Yang Qin-huan, Lei Wan-xue, Li Zheng-jun, He Zhuang-zhi, Lin Fang, Zhang Ting-you
The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Mar;26(3):444-7.
Nano-fumed silica reacted with gamma-chlopropyltrimethoxysilane as a coupling agent, and then by quaternization with N,N-dimethyl-n-tetradecylamine, finally the nano-fumed silica derivative with quaternary ammonium salts was obtained. The nano-fumed silica derivative was investigated by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), and Zeta sizer nano series. The antimicrobial properties of the nano-fumed silica derivative against selected microorganisms were tested by the quantitative suspension method. The results revealed that (1) gamma-chlopropyltrimethoxysilane can be bound to the surface of the nano-fumed silica With increasing the amount of gamma-chlopropyltrimethoxysilane, the amount of surface hydroxyl groups of nano-fumed silica decreases. (2) The Zeta potential showed that the isoelectric point of the nano-fumed silica and the modified nano-fumed silica is pH 4.8 and pH 10.5, respectively. The shift of the isoelectric point is due to the quaternary ammonium groups. (3) The obtained nano-fumed silica derivative has significant inhibition effect on the growth of microorganisms, and the bactericidal rates in 15 min for E. coil (8099), S. aureus (ATCC6538) and C. albicans(ATCC10231) were 99.99%,, 99.99% and 94.12%, respectively.
纳米气相二氧化硅与γ-氯丙基三甲氧基硅烷作为偶联剂发生反应，然后用N，N-二甲基正十四烷基胺进行季铵化反应，最终得到带有季铵盐的纳米气相二氧化硅衍生物。通过傅里叶变换红外光谱（FTIR）、差示扫描量热仪（DSC）和Zeta粒度分析仪纳米系列对该纳米气相二氧化硅衍生物进行了研究。采用定量悬浮法测试了该纳米气相二氧化硅衍生物对选定微生物的抗菌性能。结果表明：（1）γ-氯丙基三甲氧基硅烷能够结合到纳米气相二氧化硅的表面。随着γ-氯丙基三甲氧基硅烷用量的增加，纳米气相二氧化硅表面羟基的数量减少。（2）Zeta电位表明，纳米气相二氧化硅和改性纳米气相二氧化硅的等电点分别为pH 4.8和pH 10.5。等电点的移动是由于季铵基团的存在。（3）所制备的纳米气相二氧化硅衍生物对微生物的生长具有显著的抑制作用，对大肠杆菌（8099）、金黄色葡萄球菌（ATCC6538）和白色念珠菌（ATCC10231）在15分钟内的杀菌率分别为99.99%、99.99%和94.12%。
