Gentile V, Saydak M, Chiocca E A, Akande O, Birckbichler P J, Lee K N, Stein J P, Davies P J
Department of Pharmacology, University of Texas Medical School, Houston 77225.
J Biol Chem. 1991 Jan 5;266(1):478-83.
The deduced amino acid sequences for tissue transglutaminases from human endothelial cells and mouse macrophages have been derived from cloned cDNAs. Northern blot analysis of both tissue transglutaminases shows a message size of approximately 3.6-3.7 kilobases. The molecular weights calculated from the deduced amino acid sequences were 77,253 for human endothelial tissue transglutaminase and 76,699 for mouse macrophage tissue transglutaminase. The deduced amino acid sequence for the human endothelial transglutaminase was confirmed by comparison with the amino acid sequence obtained by cyanogen bromide digestion of the human erythrocyte transglutaminase. The amino acid sequences of both human endothelial and mouse macrophage tissue transglutaminases were compared to other transglutaminases. A very high degree of homology was found between human endothelial, mouse macrophage, and guinea pig liver tissue transglutaminase (greater than 80%). Moreover, human endothelial tissue transglutaminase was compared with human Factor XIIIa and a very high degree of homology (75% identity) was found in the active site region.
已从克隆的cDNA中推导得到人内皮细胞和小鼠巨噬细胞组织转谷氨酰胺酶的氨基酸序列。对这两种组织转谷氨酰胺酶进行的Northern印迹分析显示，其信使核糖核酸大小约为3.6 - 3.7千碱基。根据推导的氨基酸序列计算得出，人内皮组织转谷氨酰胺酶的分子量为77,253，小鼠巨噬细胞组织转谷氨酰胺酶的分子量为76,699。通过将人内皮转谷氨酰胺酶推导的氨基酸序列与经溴化氰消化人红细胞转谷氨酰胺酶获得的氨基酸序列进行比较，证实了该推导序列。将人内皮和小鼠巨噬细胞组织转谷氨酰胺酶的氨基酸序列与其他转谷氨酰胺酶进行了比较。发现人内皮、小鼠巨噬细胞和豚鼠肝脏组织转谷氨酰胺酶之间具有非常高的同源性（大于80%）。此外，将人内皮组织转谷氨酰胺酶与人凝血因子XIIIa进行比较，发现在活性位点区域具有非常高的同源性（75%的同一性）。
